Anti-SPON2 Rabbit Polyclonal Antibody for WB, IHC-P, ELISA - Q9BUD6

Core Product Specifications and Parameters

Biological Background: SPON2 (Spondin-2) Function and Localization

• SPON2 (Spondin-2), also known as Mindin or DIL-1, is a secreted extracellular matrix protein belonging to the spondin family (UniProt: Q9BUD6).
• It functions as a cell adhesion molecule, promoting the adhesion and outgrowth of hippocampal embryonic neurons.
• SPON2 acts as a pattern-recognition receptor, binding directly to bacteria and bacterial lipopolysaccharide (LPS), and serves as an opsonin to enhance macrophage phagocytosis.
• It plays an essential role in the initiation of the innate immune response, representing a unique pattern-recognition molecule in the extracellular matrix.
• Localized to the extracellular space and extracellular matrix, SPON2 is secreted and contains a signal peptide, disulfide bonds, and glycosylation sites.
• The protein exhibits tissue-specific expression; it is found in normal lung tissue but is absent in lung carcinoma cell lines, suggesting a potential role in cancer biology.
• Keywords associated with SPON2 include calcium binding, cell adhesion, disulfide bond, glycoprotein, immunity, innate immunity, and metal-binding.

Experimental Guidance and Technical Tips

• The immunogen corresponds to the N-terminal region (aa 27-150) of human SPON2, which may be conserved in rat, enabling cross-reactivity.
• For Western blot (WB), the predicted molecular weight is 36kDa, but consider potential glycosylation or other post-translational modifications that may affect migration.
• For immunohistochemistry (IHC-P), validate in normal lung tissue as a positive control, given specific expression in lung.
• For ELISA, SPON2 is a secreted protein, so consider using cell culture supernatants or biological fluids as samples.
• As a polyclonal antibody, lot-to-lot consistency should be verified; consider employing a blocking peptide or recombinant protein to confirm specificity.
• When studying innate immunity, combine with macrophage phagocytosis assays; the antibody may be useful for detecting SPON2 in extracellular matrix or opsonized bacteria.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio specializes in providing comprehensive IVD raw material solutions for research and diagnostic development. For SPON2-related studies in innate immunity and cell adhesion, we offer validated antibody pairs, bulk ancillary reagents, and customized sourcing of rare targets to accelerate your projects from concept to clinic. Contact us for your one-stop procurement needs in immunology and infectious disease research.