Anti-SPOCK3 Polyclonal Antibody for WB, ELISA - Q9BQ16

Core Product Specifications and Parameters

Biological Background: SPOCK3 (Testican-3) Function and Localization

• SPOCK3, also known as Testican-3, is a secreted proteoglycan belonging to the SPARC/osteonectin, CWCV, and Kazal-like domain family (SPOCK). It encodes a signal peptide, a Kazal-like domain, and an extracellular calcium-binding domain.
• The protein is primarily expressed in the brain, suggesting a role in neural tissues (tissue specificity from UniProt).
• Testican-3 participates in diverse steps of neurogenesis, potentially influencing neuronal development (UniProt FUNCTION).
• It functions as an inhibitor of matrix metalloproteinase (MMP) processing, specifically interfering with the activation of pro-MMP-2 by MT1-MMP and MT3-MMP, which may suppress tumor invasion.
• SPOCK3 is localized to the extracellular space and extracellular matrix, consistent with its role in modulating pericellular proteolysis.
• Post-translational modifications include glycosylation and potential heparan sulfate attachment, as indicated by keywords "Glycoprotein" and "Heparan sulfate".
• The protein contains disulfide bonds and calcium-binding sites, and alternative splicing produces multiple isoforms (UniProt KEYWORDS).

Experimental Guidance and Technical Tips

• The immunogen comprises a recombinant fragment (amino acids 180-380) of human SPOCK3, which includes conserved regions. For Western blot, consider using brain tissue lysates or cell lines expressing SPOCK3, as it is brain-specific.
• For ELISA applications, we suggest titrating the antibody against recombinant SPOCK3 protein, as well as testing in biological matrices (e.g., cerebrospinal fluid or cell culture supernatant) to assess native protein detection.
• Mouse and rat cross-reactivity is reported; validate the antibody in these species under your experimental conditions to confirm specificity.
• Optimal working concentrations for Western blot and ELISA should be determined empirically by the end user.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers a comprehensive portfolio of IVD raw materials, from validated antibodies to bulk ancillary reagents, supporting diagnostic manufacturers and research laboratories from concept to clinic. The SPOCK3 Rabbit pAb is part of our extensive catalog for neuroscience and oncology research, and our team can also assist in sourcing custom antibodies, matched pairs, or rare-target reagents to accelerate your assay development.