Anti-SPIN2B Rabbit Polyclonal Antibody for WB and ELISA - Q9BPZ2

Core Product Specifications and Parameters

Biological Background: SPIN2B Function and Localization

• SPIN2B (Spindlin-2B) is a member of the spindlin family of proteins, characterized by a Tudor-like domain; it is also known as SPIN-2 or TDRD26.
• It functions in cell cycle regulation by inhibiting apoptosis when essential growth factors are removed (PubMed:12145692). Related references: PMID:12145692
• The protein specifically binds to trimethylated lysine 4 on histone H3 (H3K4me3), suggesting a role in chromatin regulation (PubMed:29061846). Related references: PMID:29061846
• SPIN2B localizes predominantly to the nucleus.
• It is expressed in a variety of tissues, with highest levels detected in the liver, followed by heart, stomach, kidney, skeletal muscle, placenta, and pancreas.
• Other keywords associated with SPIN2B include apoptosis, cell cycle, nucleus, and 3D-structure (indicating available structural data), reflecting its functional roles.

Experimental Guidance and Technical Tips

• This antibody was generated using an immunogen corresponding to amino acids 1-258 of human SPIN2B (NP_001006682.1). This region covers the full-length protein, so the antibody may recognize full-length SPIN2B; however, empirical validation in the target sample is advised.
• For Western blot (WB) analysis, follow standard polyclonal antibody protocols. A typical starting dilution range is 1:500–1:2000, but optimal conditions must be determined experimentally.
• For ELISA, the antibody can be used as a capture or detection reagent; appropriate controls and assay optimization are recommended.
• Given the cross-reactivity with mouse and rat, this antibody may be suitable for studies in these species; however, sequence homology should be verified, and species-specific validation is necessary.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves as a comprehensive one-stop sourcing partner for IVD raw materials, supporting diagnostic development from concept to clinic. This SPIN2B antibody, targeting a key regulator of cell cycle and apoptosis, is part of our broad portfolio of validated antibodies and ancillary reagents. We offer tailored solutions including antibody pairs, monoclonal and polyclonal antibodies, and bulk reagent supply to accelerate your research and diagnostic applications.