Anti-SPIN1 Polyclonal Antibody for WB, IHC-P, ELISA - Q9Y657

Core Product Specifications and Parameters

Biological Background: SPIN1 Function and Localization

• Encoded by the SPIN1 gene, Spindlin-1 is a chromatin reader protein also known as Ovarian cancer-related protein, belonging to the SPIN family.
• Specifically recognizes and binds histone H3 trimethylated at both Lys-4 and Lys-9 (H3K4me3K9me3), playing a critical role in piRNA-mediated retrotransposon silencing during spermatogenesis. Related references: PMID:33574238
• Key initiator of the PIWIL4-piRNA pathway in male embryonic germ cells, promoting DNA methylation and silencing of young LINE1 transposons by recruiting PIWIL4 and SPOCD1.
• Also recognizes H3K4me3 and H3R8me2a, acting as an activator of the Wnt signaling pathway downstream of PRMT2. Related references: PMID:22258766, PMID:29061846
• In cancer, promotes cell proliferation via Wnt signaling activation; overexpression induces metaphase arrest and chromosomal instability. Related references: PMID:24589551
• Localizes to the nucleus and nucleolus, where it associates with active rDNA loci and promotes rRNA gene expression. Related references: PMID:21960006
• May participate in cell-cycle regulation during the gamete-to-embryo transition and in oocyte meiotic resumption.
• Highly expressed in ovarian cancer tissues; post-translationally modified by acetylation, phosphorylation, and isopeptide bond formation, with functional roles in meiosis and chromatin regulation.

Experimental Guidance and Technical Tips

• The immunogen is a recombinant fusion protein covering amino acids 1–262 of human SPIN1, suggesting recognition of the full-length protein; consider that native conformations may be detected.
• For Western blot, a band near 30 kDa is expected; validate with appropriate loading controls and confirm band size in your sample system.
• For IHC-P, antigen retrieval conditions (e.g., heat-induced epitope retrieval at pH 6 or 9) should be optimized based on tissue type and fixation method.
• For ELISA, the antibody can be used as a capture or detection reagent; perform checkerboard titrations to determine optimal dilutions.
• Cross-reactivity with mouse and rat indicates conserved epitopes; validate reactivity in the relevant species’ tissue or cell lysates.
• Due to the nuclear localization of SPIN1, ensure efficient nuclear protein extraction for Western blot or appropriate permeabilization for immunostaining.
• Multiple post‑translational modifications (acetylation, phosphorylation, ubiquitination‑like) may affect antibody binding; consider phosphatase or deacetylase treatment if band shifts are observed.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio enables diagnostic manufacturers and researchers to accelerate assay development by providing one‑stop access to IVD raw materials, such as this anti-SPIN1 polyclonal antibody for epigenetic and cancer research. Our portfolio includes validated antibody pairs, optimized monoclonal/polyclonal antibodies, bulk ancillary reagents, and support for sourcing rare‑target raw materials, all backed by technical consultation from concept to clinical application.