Anti-Spike Glycoprotein Monoclonal Antibody for WB, IF/ICC, ELISA - P0DTC2

Core Product Specifications and Parameters

Biological Background: Spike Glycoprotein Function and Localization

• Spike glycoprotein (S) is a class I viral fusion protein responsible for mediating SARS-CoV-2 entry into host cells.
• The S protein attaches to the host cell by interacting with the angiotensin-converting enzyme 2 (ACE2) receptor, initiating infection (PubMed:32142651, PubMed:32155444, PubMed:33607086). Related references: PMID:32142651, PMID:32155444, PMID:33607086
• Proteolytic cleavage at the S2/S2' site by host proteases TMPRSS2 or cathepsin L triggers irreversible conformational changes, leading to fusion of the viral and cellular membranes (PubMed:32142651, PubMed:32703818, PubMed:34159616, PubMed:34561887). Related references: PMID:32142651, PMID:32703818, PMID:34159616, PMID:34561887
• Alternative host entry receptors include neuropilin-1/2 (NRP1/NRP2) and asialoglycoprotein receptor 1 (ASGR1), which may explain tropism for olfactory epithelial cells and ACE2-independent pathways (PubMed:33082294, PubMed:33082293, PubMed:34837059). Related references: PMID:33082294, PMID:33082293, PMID:34837059
• The protein contains coiled coil regions, disulfide bonds, and is heavily glycosylated, consistent with its role as a viral envelope fusion glycoprotein.
• Subcellular localization includes the virion membrane, host endoplasmic reticulum-Golgi intermediate compartment, and host cell membrane.
• It contributes to viral immune evasion by inhibiting host innate immune responses and counteracting tetherin (BST-2) restriction.
• The stalk domain of S contains three hinges, providing unexpected orientational freedom to the head domain, likely aiding receptor binding (PubMed:32817270). Related references: PMID:32817270

Experimental Guidance and Technical Tips

• The immunogen was raised against a recombinant fragment of the 3CLpro protein; potential cross-reactivity with the spike glycoprotein (P0DTC2) should be validated in your specific assay context.
• For Western blotting (WB), a band near 141 kDa is expected under reducing conditions; optimize sample preparation and antibody dilution to minimize nonspecific binding.
• For immunofluorescence (IF/ICC), consider using 4% paraformaldehyde fixation and 0.1% Triton X-100 permeabilization; include appropriate controls to confirm staining specificity.
• For ELISA applications, titrate both the antibody and antigen to achieve a robust signal-to-noise ratio; cross-reactivity with other coronaviral proteins may need evaluation.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is your integrated sourcing partner for IVD raw materials, offering validated antibody pairs, optimized monoclonal and polyclonal antibodies, and bulk ancillary reagents. This anti-SARS-CoV-2 spike antibody supports viral pathogenesis research and diagnostic assay development. We also specialize in rare-target raw-material procurement and technical consulting to help accelerate your project from concept to clinic.