Anti-SPICE1 Polyclonal Antibody for Western Blot and ELISA - Q8N0Z3

Core Product Specifications and Parameters

Biological Background: SPICE1 Function and Localization

• SPICE1 (Spindle and centriole-associated protein 1), also known as coiled-coil domain-containing protein 52 (CCDC52), is a key regulator of centrosome and spindle function.
• It is essential for centriole duplication, ensuring proper bipolar spindle formation and accurate chromosome congression during mitosis.
• The protein localizes to mitotic structures, including centrosomes, centrioles, and the mitotic spindle, highlighting its role in cell division.
• SPICE1 is classified as a phosphoprotein, suggesting its activity may be modulated by cell cycle-dependent kinases.
• The predicted molecular weight is approximately 96 kDa, consistent with the observed band in Western blot applications.
• The presence of coiled-coil domains indicates potential for protein-protein interactions critical for its scaffolding functions.
• As a reference proteome member, SPICE1 is a reliable target for studying cell cycle regulation and centrosome biology.

Experimental Guidance and Technical Tips

• For Western blotting, consider using whole cell lysates from human, mouse, or rat cell lines; the expected band is ~96 kDa. Optimize gel percentage and transfer conditions accordingly.
• In ELISA, the antibody can be used to capture the recombinant immunogen or endogenous SPICE1 from cell lysates; validate with appropriate positive and negative controls.
• Given its role in mitosis, synchronizing cells at specific cell cycle stages may enhance detection sensitivity.
• As a polyclonal antibody, batch-to-batch consistency should be verified for critical assays, and optimal working dilutions need empirical determination.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers, labs, and research institutes with one-stop access to IVD raw materials, supporting the entire development cycle from concept to clinic. This anti-SPICE1 polyclonal antibody is part of our portfolio for cell division and mitosis research, offering reliable solutions for assay development. We supply optimized antibodies, validated antibody pairs, and bulk ancillary reagents, helping you efficiently source rare targets and reduce supply chain complexity.