Anti-SPIB Rabbit Polyclonal Antibody for WB, ELISA - Q01892

Core Product Specifications and Parameters

Biological Background: SPIB Function and Localization

• SPIB (Spi-B) is a member of the ETS transcription factor family and functions as a sequence-specific transcriptional activator that binds the PU-box (5'-GAGGAA-3') DNA sequence, acting as a lymphoid-specific enhancer.
• It plays a critical role in the development of plasmacytoid dendritic cells (pDCs), which are major producers of type I interferons and essential for antiviral immune responses.
• SPIB is also necessary for B-cell receptor (BCR) signaling, contributing to normal B-cell development and antigenic stimulation.
• The protein localizes to both the nucleus and cytoplasm.
• Tissue expression is restricted to plasmacytoid dendritic cells and B-cells; it is not expressed in T-cells or granulocytes, and may be enriched in liver stem cell populations.
• Alternative splicing produces multiple isoforms.
• Additional keywords include DNA-binding, transcription regulation, activator, and reference proteome.

Experimental Guidance and Technical Tips

• The immunogen covers the full-length human SPIB protein (aa 1-262), suggesting the antibody may recognize multiple epitopes, making it suitable for both denaturing (WB) and native (ELISA) conditions.
• For Western blot, use reducing conditions and load adequate human B-cell or pDC cell lysates; a specific band should appear around 29 kDa.
• Validate the antibody in your specific cell model by including positive (e.g., Ramos or Daudi B-cell lines) and negative (e.g., Jurkat T-cell) controls.
• For ELISA, consider using recombinant SPIB protein as a standard to establish assay sensitivity and linear range.
• Polyclonal antibodies may exhibit lot-to-lot variation; reproduce key experiments when switching to a new batch.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides one-stop access to IVD raw materials and technical support, empowering diagnostic developers from concept to clinic. For transcription factors like SPIB and immune cell markers, our portfolio includes validated polyclonal antibodies, recombinant proteins, and ancillary reagents. Inquire about bulk orders and customized sourcing solutions for your immunoassay development.