Anti-SPI1 Polyclonal Antibody for WB, IHC-P, ELISA - P17947

Core Product Specifications and Parameters

Biological Background: SPI1 Function and Localization

• Pioneers hematopoietic transcription by decompacting stem cell heterochromatin, enabling access for other transcription factors, and directly controlling gene expression through genetic regulatory elements (PubMed:23658224, PubMed:33951726). Related references: PMID:23658224, PMID:33951726
• Binds via its ETS domain to the purine-rich DNA core sequence 5'-GAGGAA-3' (PU-box) to regulate target genes (PubMed:33951726). Related references: PMID:33951726
• Transactivates genes critical for myeloid and lymphoid lineage commitment, such as CSF1R.
• In vitro, can also bind RNA and interfere with pre-mRNA splicing.
• Localizes to the nucleus to execute transcriptional regulatory functions.
• Highly expressed in bone marrow hematopoietic stem cells, lymphoid progenitors, myeloid lineages, and pre-B1 cells.
• Also found in germinal center B-cells.

Experimental Guidance and Technical Tips

• The immunogen spans amino acids 17-98 of human SPI1, targeting the N-terminal region; this may allow detection of full-length SPI1.
• For Western blot, a specific band around 31 kDa is expected; use appropriate positive and negative controls to validate reactivity.
• For IHC-P, optimize antigen retrieval conditions and validate staining in tissues known to express PU.1.
• Cross-reactivity with both human and mouse SPI1 is indicated; verify species-specific performance in your model system.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers and research labs with direct access to high-quality IVD raw materials like this anti-SPI1 polyclonal antibody, supporting studies of hematopoietic transcription factors. Our one-stop offering includes validated antibody pairs, optimized monoclonal/polyclonal antibodies, bulk ancillary reagents, and assistance in sourcing rare-target raw materials. For transcription factor research and beyond, connect with us to streamline your supply chain.