Anti-SPHK2 Polyclonal Antibody for WB, ELISA - Q9NRA0

Core Product Specifications and Parameters

Biological Background: SPHK2 Function and Localization

• Catalyzes the phosphorylation of sphingosine to form sphingosine-1-phosphate (SPP), a lipid mediator with intra- and extracellular functions; also acts on other sphingoid bases and binds phosphoinositides. Related references: PMID:12954646, PMID:19168031
• Unlike prosurvival SPHK1, SPHK2 positively regulates intracellular ceramide levels, inhibits cell growth, and enhances apoptosis. Related references: PMID:16118219
• In mitochondria, SPHK2-produced SPP binds PHB2 to modulate cytochrome-c oxidase assembly and mitochondrial respiration. Related references: PMID:20959514
• In the nucleus, SPHK2 interacts with HDAC1/HDAC2, inhibiting their deacetylase activity via SPP, leading to increased acetylation of histones H3-K9, H4-K5, and H2B-K12, thereby regulating epigenetic gene expression. Related references: PMID:19729656
• Nuclear SPHK2 may also inhibit DNA synthesis and cell cycle progression. Related references: PMID:12954646, PMID:16103110
• Localizes to multiple cellular compartments, including cytoplasm, nucleus, endoplasmic reticulum, mitochondrial inner membrane, and lysosome membrane.
• Predominantly expressed in kidney, liver, and brain, with isoform 1 being the major form in most tissues; detected in cerebral cortex and hippocampus at the protein level. Related references: PMID:10751414, PMID:29615132, PMID:16103110
• Annotated as an ATP-binding kinase involved in lipid metabolism; features alternative splicing and phosphorylation modifications.

Experimental Guidance and Technical Tips

• The immunogen covers the N-terminal region (aa 1-165) of human SPHK2, a domain conserved across isoforms; the antibody may recognize multiple splice variants.
• For Western blot (WB), consider using lysates from cell lines or tissues known to express SPHK2, such as HEK293, HeLa, or kidney/liver/brain samples, with appropriate loading controls.
• In ELISA, optimize the coating antibody concentration and buffer conditions according to your plate format and detection system; include a standard curve for quantification.
• Because SPHK2 associates with membranes, include mild detergents in lysis buffers to ensure efficient extraction and detection.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio simplifies IVD raw-material procurement by offering validated antibody pairs, optimized polyclonals like this anti-SPHK2 antibody, and bulk ancillary reagents for lipid metabolism and apoptosis research. Our global supply network covers rare target raw materials, ensuring consistent quality for diagnostic developers and researchers. Partner with CamelBio to accelerate your project from concept to clinic with reliable, one-stop sourcing solutions.