Anti-SPHK2 Polyclonal Antibody for IF/ICC and ELISA - Q9NRA0

Core Product Specifications and Parameters

Biological Background: Sphingosine Kinase 2 (SPHK2) Function and Localization

• Sphingosine kinase 2 (SPHK2) catalyzes the phosphorylation of sphingosine to sphingosine-1-phosphate (SPP), a bioactive lipid mediator, and also acts on dihydrosphingosine and threo-dihydrosphingosine; it binds phosphoinositides. Related references: PMID:12954646, PMID:19168031

• Unlike the pro-survival sphingosine kinase 1 (SPHK1), SPHK2 elevates cellular ceramide levels, inhibits cell growth, and promotes apoptosis. Related references: PMID:16118219

• In mitochondria, SPHK2 is crucial for cytochrome-c oxidase assembly and respiration; mitochondrial SPP binds PHB2 to regulate complex IV. Related references: PMID:20959514

• Within the nucleus, SPHK2 modulates epigenetic marks by interacting with HDAC1/2 and inhibiting their deacetylase activity, thereby upregulating histone H3-K9, H4-K5, and H2B-K12 acetylation. Related references: PMID:19729656

• Nuclear SPHK2 may also suppress DNA synthesis and cell cycle progression. Related references: PMID:12954646, PMID:16103110

• In mast cells, SPHK2 is the primary source of SPP that triggers calcium influx, NF-κB activation, and cytokine release (TNF, IL6).

• SPHK2 localizes to the cytoplasm, nucleus, endoplasmic reticulum, mitochondrial inner membrane, and lysosome membrane.

• Highest expression in adult kidney, liver, and brain; isoform 1 predominates in most tissues. Related references: PMID:10751414, PMID:29615132, PMID:16103110

• The protein is annotated with keywords: alternative splicing, kinase, lipid metabolism, phosphoprotein, and reference proteome.

Experimental Guidance and Technical Tips

• Since the immunogen corresponds to the C-terminal region (aa 480-654), this antibody may recognize both full-length SPHK2 and C-terminal fragments. Consider using peptide blocking to confirm specificity in IF/ICC.

• Optimize fixation and permeabilization for IF/ICC to preserve the mitochondrial or nuclear localization of SPHK2.

• For ELISA, use recombinant SPHK2 or cell lysates from human kidney, liver, or brain tissues where SPHK2 is highly expressed.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers, labs, and research institutes with one-stop access to IVD raw materials, technical services, and consulting—covering every stage from concept to clinic. For sphingolipid signaling and apoptosis research, we offer validated antibody pairs, optimized polyclonal antibodies like this SPHK2 Rabbit pAb, bulk ancillary reagents, and support for rare target raw-material sourcing.