Anti-SPHK1 Rabbit Monoclonal Antibody for WB and ELISA - Q9NYA1

Core Product Specifications and Parameters

Biological Background: SPHK1 Function and Localization

• SPHK1 (sphingosine kinase 1), also known as SK1 and acetyltransferase SPHK1, is a multifunctional enzyme encoded by the SPHK1 gene (Gene ID: 8877) in humans. It belongs to the sphingosine kinase family and plays key roles in sphingolipid metabolism and signaling.
• Catalyzes the phosphorylation of sphingosine to form sphingosine 1-phosphate (S1P), a bioactive lipid mediator with intracellular and extracellular functions. Acts specifically on D-erythro-sphingosine and sphinganine, but not on other lipids. Related references: PMID:11923095, PMID:20577214, PMID:23602659
• In contrast to proapoptotic SPHK2, negatively regulates intracellular ceramide levels, enhances cell growth, and inhibits apoptosis. Related references: PMID:16118219
• Mediates TNF signaling: via S1P, stimulates TRAF2 E3 ubiquitin ligase activity and promotes NF-kappa-B activation, leading to IL-17 secretion. Also negatively regulates RANTES induction through the p38 MAPK pathway in response to TNF. Related references: PMID:20577214, PMID:23935096
• Exhibits serine acetyltransferase activity on PTGS2/COX2 in an acetyl-CoA-dependent manner, influencing neuronal secretion of specialized preresolving mediators (SPMs) like 15-R-lipoxin A4 during neuroinflammation.
• Localizes to the cytoplasm, nucleus, cell membrane, endosome membrane, clathrin-coated pits, and synapses, reflecting its diverse roles in signaling and membrane trafficking.
• Widely expressed, with highest levels in adult liver, kidney, heart, skeletal muscle, and brain cortex. Related references: PMID:29662056
• Contains ATP-binding and calmodulin-binding domains; regulated by phosphorylation and alternative splicing (UniProt keywords: Kinase, Transferase, Phosphoprotein, Alternative splicing).

Experimental Guidance and Technical Tips

• The immunogen was designed against the N-terminal region (aa 1-160) of human SPHK1 (NP_001136074.1). This region is common to multiple isoforms and may provide broad detection; verify sequence alignment for isoform-specific needs.
• For Western blot, a band near 43 kDa is expected, but post-translational modifications or alternative splicing may result in band shifts. Optimize loading controls and antibody dilution using relevant positive and negative lysates.
• For ELISA, consider pairing with capture/detection antibodies validated for SPHK1. Validate assay specificity using recombinant protein and appropriate negative controls.
• Use tissue or cell lysates with known SPHK1 expression (e.g., liver, kidney, or brain cortex) as positive controls, and include a negative control (e.g., SPHK1 knockdown or tissue with low expression).

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers, research laboratories, and academic institutes with one-stop access to IVD raw materials, technical services, and consulting—from concept to clinic. As your sourcing partner for sphingolipid metabolism and inflammation research, we supply validated antibody pairs, optimized monoclonal and polyclonal antibodies, bulk ancillary reagents, and rare target raw-material sourcing. Whether you need custom antibody development or consistent large-scale supply, CamelBio bridges your IVD development requirements.