Anti-SPHK1 Polyclonal Antibody for IHC-P, ELISA - Q9NYA1

Core Product Specifications and Parameters

Biological Background: SPHK1 Function and Localization

• SPHK1, also known as SPHK, is a sphingosine kinase that catalyzes the phosphorylation of sphingosine to form sphingosine 1-phosphate (S1P), a key lipid mediator with intra- and extracellular functions (PubMed:11923095, PubMed:20577214, PubMed:23602659). Related references: PMID:11923095, PMID:20577214, PMID:23602659
• In contrast to proapoptotic SPHK2, SPHK1 enhances cell growth and inhibits apoptosis, and it also participates in endocytic membrane trafficking and endosomal maturation independently of its kinase activity (PubMed:16118219, PubMed:24929359, PubMed:28049734). Related references: PMID:16118219, PMID:24929359, PMID:28049734
• Through S1P, SPHK1 stimulates TRAF2 E3 ubiquitin ligase activity and promotes NF-kappa-B activation in response to TNF signaling, leading to IL-17 secretion; it also negatively regulates RANTES induction via the p38 MAPK pathway (PubMed:20577214, PubMed:23935096). Related references: PMID:20577214, PMID:23935096
• Additionally, SPHK1 has serine acetyltransferase activity on PTGS2/COX2, which is enhanced by sphingosine, and promotes neuronal secretion of specialized pro-resolving mediators during neuroinflammation.
• Subcellularly, SPHK1 localizes to the cytoplasm, nucleus, cell membrane, endosome membrane, clathrin-coated pits, and synapse.
• SPHK1 is widely expressed, with highest levels in adult liver, kidney, heart, and skeletal muscle, and is also detected in brain cortex at the protein level.
• Keywords highlight its roles in lipid metabolism, calmodulin binding, phosphorylation, and alternative splicing.

Experimental Guidance and Technical Tips

• The immunogen used is a synthetic peptide encompassing amino acids 201-300 of human SPHK1 (NP_001136074.1). Polyclonal antibodies generated against this region may recognize multiple isoforms or post-translationally modified forms; researchers should verify specificity in their experimental context.
• For IHC-P, consider using tissues with high SPHK1 expression (e.g., liver or kidney) as positive controls, and optimize antigen retrieval and antibody dilution empirically.
• In ELISA, the antibody may be used to detect SPHK1 in cell lysates or recombinant protein preparations; appropriate blocking and washing conditions should be determined.
• Cross-reactivity has been reported with mouse and rat, but sequence homology should be checked for the immunogen region in these species to confirm expected recognition.
• As a polyclonal antibody, it may be suitable for detecting denatured or native SPHK1, but denaturing Western blot conditions may not be optimal given the application limitations; if attempting other applications, validation is required.

CamelBio: Your One-Stop Sourcing Bridge

As a one-stop partner for IVD raw materials, CamelBio offers this SPHK1 antibody alongside a comprehensive portfolio of validated antibody pairs, monoclonal and polyclonal antibodies, bulk ancillary reagents, and rare target sourcing. Whether your research focuses on sphingosine kinase 1 signaling in cancer, inflammation, or neuroinflammation, we provide efficient procurement solutions to support your diagnostic or academic projects from concept to clinic.