Anti-Spermine synthase Monoclonal Antibody for WB, ELISA - P52788

Core Product Specifications and Parameters

Biological Background: Spermine synthase Function and Localization

• Spermine synthase (SMS), also known as spermidine aminopropyltransferase, is a key enzyme in polyamine biosynthesis.
• It catalyzes the production of spermine from spermidine and decarboxylated S-adenosylmethionine (dcSAM), a critical step for normal cell growth and function.
• SMS belongs to the aminopropyltransferase family and shares structural features with other polyamine biosynthetic enzymes.
• The protein is subject to post-translational modifications, including acetylation and phosphorylation, which may regulate its activity or stability.
• Multiple isoforms exist due to alternative splicing, potentially contributing to tissue-specific regulation.
• Mutations in the SMS gene are linked to intellectual disability, highlighting its importance in neuronal development.
• As a transferase, SMS operates in the cytoplasm, although specific subcellular localization details are context-dependent.

Experimental Guidance and Technical Tips

• The immunogen used is a synthetic peptide spanning residues 50–150 of human SMS; this region exhibits high conservation across human, mouse, and rat, supporting the observed cross-reactivity.
• For western blotting, consider using standard rabbit monoclonal antibody protocols; validate dilution and incubation conditions in your specific sample type.
• In ELISA applications, direct or sandwich formats may be tested; consider using recombinant SMS protein or the immunizing peptide as a coating antigen for optimization.
• Endogenous SMS expression may vary across cell lines and tissues; include appropriate positive and negative controls to confirm specificity.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers comprehensive IVD raw material solutions, including optimized monoclonal antibodies like this anti-Spermine synthase clone, to support diagnostic assay development. Our portfolio encompasses validated antibody pairs, bulk ancillary reagents, and targeted sourcing for rare or specialized targets in polyamine metabolism and related research areas. Partner with us to streamline your workflow from concept to clinic.