Anti-SPEM1 Polyclonal Antibody for WB, ELISA - Q8N4L4

Core Product Specifications and Parameters

Biological Background: SPEM1 Function and Localization

• SPEM1 (Spermatid maturation protein 1), also known as C17orf83, is a developmental protein essential for spermatogenesis.
• It plays a critical role in the removal of excess cytoplasm during spermatid maturation, a key step in sperm formation.
• The protein contains a transmembrane helix and coiled-coil domain, suggesting membrane-associated functions and potential protein-protein interactions.
• Subcellularly, SPEM1 localizes to both the membrane and cytoplasm, consistent with its role in cytoplasm removal.
• Classified as a reference proteome member with confirmed protein identity through mass spectrometry-based proteomics (Proteomics identification).
• Its differentiation-specific expression links SPEM1 to the developmental pathway of male germ cell maturation.

Experimental Guidance and Technical Tips

• The immunogen design targeting the C-terminal region (aa203-309) of human SPEM1 ensures specific recognition; cross-reactivity with rat suggests conserved epitopes—validate in the relevant sample system.
• For Western blotting, consider using whole-cell lysates from testicular tissues or SPEM1-expressing cell lines; expect a band at approximately 35 kDa.
• In ELISA, this polyclonal antibody can be explored as a capture or detection reagent—optimization of working concentrations and pairing is recommended.
• Due to its transmembrane and cytoplasmic localization, appropriate lysis buffers (e.g., RIPA) are advised to fully solubilize the target protein.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers, laboratories, and research institutes with a one-stop sourcing solution for IVD raw materials, offering this SPEM1 rabbit polyclonal antibody as a reliable tool for spermatogenesis and male fertility research. From validated antibody pairs to bulk ancillary reagents and rare-target raw-material sourcing, we support your entire development pipeline from concept to clinic—streamlining procurement and accelerating time to market.