Anti-SPECC1L Polyclonal Antibody for WB, IHC-P, ELISA - Q69YQ0

Core Product Specifications and Parameters

Biological Background: SPECC1L Function and Localization

• Cytospin-A (SPECC1-like protein) is encoded by the SPECC1L gene (also known as CYTSA, KIAA0376) and is also referred to as renal carcinoma antigen NY-REN-22. Alternative splicing generates multiple isoforms.
• The protein plays a key role in cytokinesis and spindle organization, contributing to proper cell division.
• SPECC1L is involved in actin cytoskeleton organization and microtubule stabilization, which are essential for cell adhesion and migration.
• It localizes to the cytoplasm, cytoskeleton, mitotic spindle, and gap junctions at the cell membrane.
• Classified as a phosphoprotein and a disease variant (associated with Teebi hypertelorism syndrome and other developmental disorders), highlighting its importance in development and signaling.
• Contains a coiled-coil domain, suggesting involvement in protein-protein interactions.

Experimental Guidance and Technical Tips

• This antibody was generated against a recombinant fusion protein spanning amino acids 200–500 of human SPECC1L. Consider whether this immunogen region aligns with your target epitope requirements.
• For Western blotting, the predicted molecular weight is approximately 125 kDa. Use an appropriate gel percentage and optimize transfer conditions for high-molecular-weight proteins.
• In IHC-P, antigen retrieval may be necessary; validate staining in known positive control tissues.
• For ELISA, titrate the antibody to determine optimal working concentrations for your specific assay format.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers and research labs with a one-stop sourcing solution for IVD raw materials. The SPECC1L Rabbit pAb is part of our portfolio of validated antibodies targeting cytoskeletal and cell-division-related proteins. Beyond this product, we offer customized sourcing for validated antibody pairs, optimized monoclonal/polyclonal antibodies, and bulk ancillary reagents to accelerate your assay development.