Anti-SPDEF Rabbit Polyclonal Antibody for WB, IHC-P, IF/ICC, ELISA - O95238

Core Product Specifications and Parameters

Biological Background: SPDEF Function and Localization

• SPDEF (Prostate-derived Ets factor), also known as PDEF, is a SAM pointed domain-containing Ets transcription factor encoded by the SPDEF gene. It belongs to the ETS family of transcription factors and contains a pointed (PNT) domain and an ETS DNA-binding domain.
• SPDEF functions as an androgen-independent transactivator of the prostate-specific antigen (PSA) promoter and binds specifically to 5'-GGAT-3' DNA sequences. This activity highlights its role in prostate epithelial regulation.
• It also acts as a transcriptional activator for the SERPINB5 (maspin) promoter, a gene implicated in tumor suppression and inhibition of metastasis.
• Tissue expression is highly restricted to hormone-regulated epithelial tissues, with the highest levels in the prostate gland. Significantly lower expression is observed in mammary gland, salivary gland, and ovary. SPDEF is also expressed in prostate carcinoma cells.
• Subcellular localization is nuclear, consistent with its function as a transcription factor.
• According to UniProt, SPDEF is subject to alternative splicing and its three-dimensional structure has been solved (3D-structure keyword). These features may influence antibody recognition and functional studies.

Experimental Guidance and Technical Tips

• The immunogen was a recombinant fusion protein covering the full-length human SPDEF (aa 1-335); the antibody may recognize multiple isoforms due to alternative splicing. Confirm specificity in your system if multiple bands appear.
• Predicted molecular weight by UniProt is ~37.5 kDa, while the supplier reports 38 kDa. Minor mobility shifts in SDS-PAGE can result from post-translational modifications or running conditions.
• This antibody is suitable for WB, IHC-P, IF/ICC, and ELISA. For IHC-P, consider optimizing antigen retrieval protocols as nuclear antigens may require stronger unmasking steps.
• Expected nuclear staining in IF/ICC; use a counterstain such as DAPI to verify subcellular localization. Validate the antibody in your specific cell line or tissue.
• Cross-reactivity with rat is indicated. For rat experiments, verify performance using positive and negative controls, as reactivity may vary between strains.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is your integrated partner for IVD raw-material sourcing, providing validated antibody pairs, optimized monoclonal and polyclonal antibodies, bulk ancillary reagents, and custom solutions to accelerate your diagnostic development. This anti-SPDEF polyclonal antibody serves as a reliable tool for prostate cancer and hormone-regulated epithelial research. Let CamelBio bridge your transition from concept to clinic with efficient, one-stop procurement.