Anti-SPDEF Polyclonal Antibody for WB, ELISA - O95238

Core Product Specifications and Parameters

Biological Background: SPDEF Function and Localization

• SPDEF (SAM pointed domain-containing Ets transcription factor), also known as PDEF or PSE, is a member of the ETS transcription factor family.
• It functions as an androgen-independent transactivator of the prostate-specific antigen (PSA) promoter and also activates the SERPINB5 promoter.
• The protein binds to 5'-GGAT-3' DNA motifs and plays a role in prostate gland regulation and prostate cancer development.
• SPDEF is primarily localized in the nucleus, consistent with its role as a transcription factor.
• It exhibits highly restricted expression, with the highest levels in the prostate gland; significantly lower expression is observed in other hormone-regulated tissues like mammary gland, salivary gland, and ovary.
• The gene undergoes alternative splicing, yielding multiple isoforms. The canonical protein has a molecular weight of approximately 38 kDa.
• SPDEF features a SAM pointed domain characteristic of ETS factors, and its 3D structure has been determined.
• Classified as a DNA-binding transcriptional activator, SPDEF has been identified in proteomics studies.

Experimental Guidance and Technical Tips

• For Western blot, expect a band around 38 kDa corresponding to the canonical human SPDEF. Validate in a relevant sample system, such as prostate carcinoma cell lines.
• The immunogen corresponds to amino acids 1-100 of human SPDEF; the antibody may recognize multiple isoforms if the epitope is conserved.
• For ELISA applications, this antibody may be used for detection of recombinant or endogenous SPDEF; consider titration and a peptide blocking control to confirm specificity.
• Cross-reactivity has been demonstrated with human samples; reactivity with other species should be tested independently.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers, labs, and research institutes with one-stop access to IVD raw materials, technical services, and consulting—covering every stage from concept to clinic. For transcription factor research and prostate cancer biomarker development, we offer validated antibody pairs, optimized polyclonal antibodies like this anti-SPDEF antibody, bulk ancillary reagents, and rare target raw-material sourcing support.