Anti-SPC25 Polyclonal Antibody for WB, ELISA - Q9HBM1

Core Product Specifications and Parameters

Biological Background: SPC25 Function and Localization

• SPC25 is a key component of the kinetochore-associated NDC80 complex, essential for chromosome segregation and spindle checkpoint activity. Related references: PMID:14699129, PMID:14738735
• It is required for kinetochore integrity and the organization of stable microtubule attachment sites at the outer kinetochore. Related references: PMID:14699129, PMID:14738735
• The NDC80 complex synergistically enhances the SKA1 complex's affinity for microtubules and facilitates tracking of depolymerizing microtubule ends. Related references: PMID:23085020
• SPC25 localizes to the nucleus, centromere, and kinetochore during cell division.
• It is a phosphoprotein involved in mitosis and cell cycle progression.
• Additional features include a coiled coil domain, available 3D-structure, and identification by proteomics.

Experimental Guidance and Technical Tips

• The immunogen corresponds to a region within the C-terminal half of human SPC25, which may contribute to specific recognition of the target.
• For Western blotting, consider using a dilution range appropriate for polyclonal antibodies and validating in human cell lines expressing SPC25.
• ELISA can be used to quantify SPC25 or screen for antibody reactivity; consider using recombinant SPC25 protein as a standard.
• This antibody is designed for human SPC25; cross-reactivity with other species has not been tested.
• The predicted molecular weight is 26 kDa; confirm band size in your experimental system.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers, labs, and research institutes with one-stop access to IVD raw materials, technical services, and consulting—covering every stage from concept to clinic. This SPC25 polyclonal antibody supports research in chromosome biology and mitosis. CamelBio offers a range of IVD raw materials including validated antibody pairs, optimized monoclonal/polyclonal antibodies, bulk ancillary reagents, and rare target sourcing. Contact us for customized support.