Anti-SPC24 Rabbit Polyclonal Antibody for WB and ELISA - Q8NBT2

Core Product Specifications and Parameters

Biological Background: SPC24 Function and Localization

• SPC24 (Spindle Pole Body Component 24, also known as SPBC24) is a kinetochore protein encoded by the SPC24 gene, with a molecular weight of approximately 22 kDa.
• As a core component of the NDC80 complex, SPC24 is essential for chromosome segregation, spindle checkpoint activity, and kinetochore integrity (PubMed:14738735). Related references: PMID:14738735
• The NDC80 complex, containing SPC24, cooperates with the SKA1 complex to enhance microtubule binding and track depolymerizing microtubules (PubMed:23085020). Related references: PMID:23085020
• SPC24 localizes to the nucleus, specifically at the centromere and kinetochore during cell division.
• SPC24 is a phosphoprotein involved in mitosis and cell cycle control, associated with keywords including alternative splicing and coiled coil.

Experimental Guidance and Technical Tips

• The immunogen covers the N-terminal region (amino acids 1-197) of human SPC24, which is likely to generate antibodies recognizing the native protein in applications such as Western blotting.
• For WB, consider using whole cell lysates from human or mouse cell lines, given the reported cross-reactivity.
• Validate the antibody in the relevant sample system, as performance may vary with experimental conditions.
• For ELISA, the polyclonal nature may provide robust detection; consider using the recombinant immunogen as a positive control.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers, labs, and research institutes with one-stop access to IVD raw materials, including validated antibody pairs, optimized monoclonal/polyclonal antibodies, and bulk ancillary reagents. Whether you need SPC24 antibodies for kinetochore research or rare target raw-material sourcing, our supply capabilities help bridge the gap from concept to clinic.