Anti-SPATA4 Rabbit Polyclonal Antibody for WB and ELISA - Q8NEY3

Core Product Specifications and Parameters

Biological Background: SPATA4 Function and Localization

• SPATA4 (Spermatogenesis-associated protein 4), also designated TSARG2 (Testis spermatocyte apoptosis-related gene 2 protein), is encoded by the SPATA4 gene.
• SPATA4 is implicated in the regulation of apoptosis, though its precise role remains to be fully characterized.
• Subcellularly, SPATA4 localizes predominantly to the nucleus.
• Expression is highly tissue-specific, with significant levels detected in the testis, particularly within seminiferous tubules.
• The protein is cataloged in the reference proteome and has been identified in large-scale proteomics studies (Keywords: Proteomics identification, Reference proteome).
• The predicted molecular weight is approximately 35 kDa (34751 Da).

Experimental Guidance and Technical Tips

• The immunogen consists of the N-terminal region (amino acids 1-250) of human SPATA4, which may confer epitope specificity for this polypeptide segment.
• For Western blot, consider using testis tissue lysates as a positive control, given the high expression in testis. Optimal dilutions and conditions should be determined empirically.
• In ELISA, the antibody may be suitable for detecting recombinant or endogenous SPATA4, but thorough validation with appropriate standards and sample matrices is advised.
• Cross-reactivity with mouse and rat SPATA4 has been reported, enabling comparative studies in rodent models.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves as a comprehensive sourcing partner for IVD raw materials, streamlining procurement from discovery to clinical application. This SPATA4 antibody supports researchers studying apoptosis and spermatogenesis, and we offer additional validated reagents, including antibody pairs and bulk monoclonal/polyclonal options. For rare targets or customized raw-material needs, our team provides dedicated sourcing and technical consultation to accelerate your project timelines.