Anti-SPARCL1 Rabbit Polyclonal Antibody for WB and ELISA - Q14515

Core Product Specifications and Parameters

Biological Background: SPARCL1 Function and Localization

• SPARCL1 (SPARC-like protein 1), also designated Hevin or MAST 9, is a secreted extracellular matrix protein.
• The protein localizes to the extracellular space and extracellular matrix (secreted).
• SPARCL1 undergoes alternative splicing, generating multiple isoforms.
• It contains calcium-binding sites and forms intramolecular disulfide bonds.
• Post-translational modifications include glycosylation and phosphorylation.
• Tissue expression is broad: highly expressed in lymph node, brain, heart, lung, skeletal muscle, ovary, small intestine, and colon; lower in placenta, pancreas, testis, spleen, and thymus; not detected in kidney, liver, and peripheral blood leukocytes.
• The reference isoform has a calculated molecular weight of approximately 75.2 kDa, consistent with the observed 75 kDa band on Western blots.

Experimental Guidance and Technical Tips

• The immunogen encompasses amino acids 17–300 of human SPARCL1; this N-terminal region may confer target specificity; validate reactivity using endogenous protein in tissue or cell lysates.
• For Western blot, expect a band at ~75 kDa in human, mouse, and rat samples; optimize blocking conditions and antibody dilution for best signal-to-noise ratio.
• For ELISA, the antibody may be used to capture or detect SPARCL1 in biological fluids, cell supernatants, or tissue extracts; pairing with a detection antibody can improve sensitivity.
• As a secreted protein, consider using serum-free conditioned media or enriched extracellular matrix preparations to enhance detection.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is a dedicated IVD raw-material partner, providing diagnostic developers and researchers with validated antibody pairs, bulk ancillary reagents, and custom sourcing for rare targets. For SPARCL1-related projects—such as extracellular matrix remodeling, fibrosis, or tumor microenvironment studies—CamelBio offers ready-to-screen monoclonal and polyclonal antibodies, along with optimized ancillary reagents, to support your assay development from concept to clinic.