Anti-SPAM1 Rabbit Polyclonal Antibody for WB, IF-P, IHC-P, ELISA - P38567

Core Product Specifications and Parameters

Biological Background: SPAM1 Function and Localization

• SPAM1 encodes hyaluronidase PH-20, also referred to as sperm adhesion molecule 1, hyaluronoglucosaminidase PH-20, and sperm surface protein PH-20.
• The protein is a glycosylphosphatidylinositol (GPI)-anchored cell membrane glycoprotein belonging to the glycoside hydrolase family, possessing hyaluronoglucosaminidase catalytic activity.
• It plays a pivotal role in fertilization by digesting hyaluronic acid, the major component of the cumulus cell extracellular matrix surrounding the oocyte, thereby facilitating sperm penetration toward the zona pellucida.
• Localized on the sperm cell membrane, SPAM1 mediates cell adhesion and recognition events during sperm-egg binding.
• Expression is predominantly restricted to testis tissue.
• Post-translational modifications include N-linked glycosylation, a GPI-anchor, and disulfide bond formation essential for structural stability; the protein also undergoes alternative splicing to generate variant isoforms.

Experimental Guidance and Technical Tips

• The immunogen corresponds to a conserved central region of human SPAM1, potentially enabling cross-reactivity with mouse and rat orthologs as indicated; however, sequence alignment should be verified for specific species variants.
• For western blotting, the predicted molecular weight is ~58 kDa; endogenous expression may be testis-specific, so appropriate tissue lysates (e.g., human, mouse, or rat testis) should be used as positive controls.
• In immunofluorescence and immunohistochemistry, permeabilization is required for intracellular epitope detection; optimal fixation and permeabilization conditions may need empirical optimization for different sample types.
• For ELISA applications, the antibody can be used as a capture or detection reagent; consider titrating the antibody concentration to establish a suitable dynamic range for the assay.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is your dedicated partner for IVD raw-material procurement, offering a comprehensive portfolio that includes validated antibody pairs, recombinant proteins, and ancillary reagents. This anti-SPAM1 polyclonal antibody, targeting a key sperm surface protein involved in fertilization, is designed to support reproductive biology research and fertility-related diagnostic development. With robust supply chain integration and technical consultation, CamelBio helps you accelerate transitions from concept to clinic.