Anti-SPAG8 Rabbit Polyclonal Antibody for WB and ELISA - Q99932

Core Product Specifications and Parameters

Biological Background: SPAG8 Function and Localization

• SPAG8, also known as SMP-1, BS-84, HSD-1, or CT142, is a sperm-associated antigen and a microtubule inner protein (MIP) component of the dynein-decorated doublet microtubules (DMTs) in cilia and flagella axonemes.
• SPAG8 is required for motile cilia beating, as demonstrated in airway epithelial cells. Related references: PMID:36191189
• In spermatogenesis, SPAG8 enhances the binding of CREM isoform tau to its coactivator FHL5, increasing FHL5-regulated transcriptional activation (By similarity).
• SPAG8 participates in the acrosome reaction and the binding of sperm to the zona pellucida during fertilization (By similarity).
• The protein regulates the cell cycle by controlling progression through the G2/M phase, possibly by delaying CDK1 activation required for mitotic entry. Related references: PMID:19548270
• SPAG8 interacts with RANBP9, a connection that may influence microtubule formation and fertility. Related references: PMID:10500252
• Subcellular localization includes the cytoplasm, nucleus, acrosome, spindle, and the axonemes of cilia and flagella, reflecting its diverse functional roles.
• Expression is predominantly in testis germ cells and airway epithelial cells; it is absent from tissues such as liver, kidney, prostate, and small intestine.

Experimental Guidance and Technical Tips

• The immunogen corresponds to residues 220–420 of human SPAG8, a region that may capture multiple isoforms; consider epitope mapping if isoform specificity is required.
• For Western blot, the predicted molecular weight is approximately 51 kDa; include positive control lysates from testis or ciliated cell lines (e.g., airway epithelial cells) to confirm reactivity.
• Given cross-reactivity with mouse and rat, the antibody can be used for comparative studies; verify species-specific background levels in your model system.
• In ELISA applications, validate detection against the recombinant immunogen or native SPAG8 from relevant biological fluids/extracts.
• As a polyclonal antibody, lot-to-lot consistency should be assessed; optimize working concentrations for each experimental setup.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves as a comprehensive sourcing partner for IVD raw materials, helping diagnostic manufacturers and research laboratories accelerate development from concept to clinic. This anti-SPAG8 polyclonal antibody targets a key player in cilia biology and fertility, areas with growing diagnostic potential. Beyond individual antibodies, CamelBio provides validated antibody pairs, optimized monoclonal/polyclonal reagents, bulk ancillary materials, and rare target sourcing to support your entire assay pipeline.