Anti-SPAG6 Rabbit Polyclonal Antibody for WB and ELISA - O75602

Core Product Specifications and Parameters

Biological Background: SPAG6 Function and Localization

• SPAG6 (Sperm-associated antigen 6), also known as PF16 homolog, Repro-SA-1, or sperm flagellar protein, is a key structural component of the sperm flagellum.
• It plays a critical role in maintaining the structural integrity of the central apparatus of the sperm axoneme, which is essential for flagellar motility and sperm function.
• Encoded by the SPAG6 gene (GeneID: 9576) in humans, this protein is evolutionarily conserved and shares homology with the Chlamydomonas PF16 protein.
• SPAG6 is predominantly expressed in the testis, consistent with its specialized function in sperm biology.
• Subcellularly, it localizes to the cytoplasm and cytoskeleton, specifically within the axoneme of motile cilia and flagella, highlighting its role in microtubule-based structures.
• The presence of multiple isoforms due to alternative splicing suggests potential functional diversity or regulation (UniProt keyword: Alternative splicing).

Experimental Guidance and Technical Tips

• For Western blot (WB), the expected band size is approximately 55 kDa (calculated MW 55.4 kDa) in human testis lysates or cell lines expressing SPAG6. Since expression is testis-specific, consider using human testis tissue extracts as a positive control.
• The antibody is a rabbit polyclonal, which may recognize multiple epitopes; validate specificity by peptide competition or knockdown/knockout controls.
• For ELISA, the recombinant immunogen can be used as a standard or coating antigen; optimize coating concentration and detection antibody dilution.
• Given the flagellar localization, this antibody may be useful for immunofluorescence in sperm or ciliated cells; however, this application has not been validated and would require optimization.

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