Anti-SPAG5 Polyclonal Antibody for IF/ICC, ELISA - Q96R06

Core Product Specifications and Parameters

Biological Background: SPAG5 Function and Localization

• SPAG5 (Sperm-associated antigen 5), also known as Astrin, Deepest, and MAP126, is a 134 kDa protein highly expressed in testis. It localizes to the cytoplasm, mitotic spindle, spindle poles, kinetochores, midbody, and centrosomes.
• Essential component of the mitotic spindle required for normal chromosome segregation and progression into anaphase. Related references: PMID:11724960, PMID:12356910, PMID:27462074
• Required for chromosome alignment, normal timing of sister chromatid segregation, and maintenance of spindle pole architecture. Related references: PMID:17664331, PMID:27462074
• In complex with SKAP, promotes stable microtubule-kinetochore attachments and may regulate separase activity.
• May regulate AURKA localization to mitotic spindle and CCNB1 localization to both spindle and centrosomes. Related references: PMID:18361916, PMID:21402792
• Involved in centriole duplication; required for centrosomal localization of CDK5RAP2, CEP152, WDR62, and CEP63, and promotes centrosomal localization of CDK2. Related references: PMID:26297806
• In non-mitotic cells, inhibits mTORC1 by recruiting RPTOR to stress granules upon stress, preventing mTORC1 hyperactivation-induced apoptosis. Related references: PMID:23953116
• May enhance GSK3B-mediated phosphorylation of substrates such as MAPT/TAU. Related references: PMID:18055457

Experimental Guidance and Technical Tips

• The immunogen is a recombinant protein covering amino acids 1-300 of human SPAG5, which may generate antibodies targeting multiple epitopes along the N-terminal region.
• For immunofluorescence (IF/ICC), consider using mitotic cells (e.g., HeLa or U2OS synchronized with nocodazole) to visualize spindle and kinetochore localization. Optimize fixation/permeabilization protocols to preserve spindle structure.
• For ELISA, recombinant SPAG5 protein or cell lysates from testis or mitotically active cells can serve as positive controls. Perform antibody titration to determine the optimal working dilution.
• Validate antibody specificity in the relevant sample system (e.g., siRNA knockdown or CRISPR knockout) to confirm specific detection of SPAG5.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides a comprehensive sourcing bridge for IVD manufacturers, offering validated antibody pairs, optimized monoclonal and polyclonal antibodies, and bulk ancillary reagents. With expertise in cell cycle and mitotic spindle targets, we connect you to rare-target raw materials and technical consultation to streamline assay development from concept to clinic. Let CamelBio be your trusted partner in securing reliable raw materials for your diagnostic workflows.