Anti-SPAG11A Polyclonal Antibody for WB, ELISA - Q6PDA7

Core Product Specifications and Parameters

Biological Background: SPAG11A Function and Localization

• Encodes SPAG11A (Sperm-associated antigen 11A), also known as Human epididymis-specific protein 2 (HE2), antimicrobial-like protein Bin-1b, or Protein EP2; classified within the beta-defensin family of host defense peptides.
• Exhibits antimicrobial activity against Escherichia coli, contributing to innate immunity in the male reproductive tract (By similarity).
• Plays a critical role in sperm maturation, storage, and protection within the epididymal environment (By similarity).
• Predominantly secreted into the extracellular space; possesses a signal peptide for secretion.
• Undergoes alternative splicing, generating multiple isoforms, and is annotated as a glycoprotein with defensin and antibiotic properties.
• Calculated molecular weight is approximately 13.8 kDa, consistent with a small secreted peptide.

Experimental Guidance and Technical Tips

• The immunogen is a synthetic peptide covering the N-terminal region (aa 1-50) of human SPAG11A; this may recognize both full-length and processed forms of the protein.
• For Western blotting, expect a band near 14 kDa; consider using epididymis or reproductive tissue lysates as positive controls and validate in the relevant sample system.
• ELISA applications may require optimization of coating and detection conditions; consider pairing with another antibody for sandwich ELISA development.
• Due to homology, cross-reactivity with mouse and rat is indicated; verify suitability for non-human models if using for cross-species studies.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio connects diagnostic manufacturers and laboratories with a one-stop supply of IVD raw materials for reproductive health and antimicrobial research. This anti-SPAG11A polyclonal antibody is available alongside validated antibody pairs, optimized monoclonal/polyclonal antibodies, bulk ancillary reagents, and rare target sourcing to accelerate your assay development from concept to clinic.