Anti-SP7 (Osterix) Rabbit Polyclonal Antibody for WB, ELISA - Q8TDD2

Core Product Specifications and Parameters

Biological Background: SP7 Function and Localization

• SP7 (transcription factor Sp7, also known as osterix) is a zinc finger transcription factor that serves as a master regulator of osteoblast differentiation and bone formation. Related references: PMID:23457570
• It functions by binding to SP1 and EKLF consensus sequences and other G/C-rich promoter elements, thereby activating osteoblast-specific genes.
• SP7 is a member of the Sp family of transcription factors, characterized by three C2H2-type zinc finger motifs that mediate DNA binding.
• The protein localizes exclusively to the nucleus, consistent with its role in transcriptional regulation.
• Expression is highly tissue-specific, restricted to bone-derived cells, which underscores its specialized role in skeletal development.
• Post-translational modifications include isopeptide bond and ubiquitination (Ubl conjugation), which may regulate its stability or activity.
• Mutations in SP7 are linked to osteogenesis imperfecta, a disorder characterized by fragile bones, reinforcing its clinical significance.
• Multiple isoforms produced by alternative splicing may modulate its transcriptional activity in different contexts.

Experimental Guidance and Technical Tips

• The immunogen corresponds to a region in the C-terminal portion of human SP7 (aa 333–376), which lies outside the zinc finger DNA-binding domain. This design may allow detection of both unbound and DNA-bound forms of the protein.
• For Western blot, expect a specific band at approximately 45 kDa. Validate antibody specificity using osteoblast-like cell lines (e.g., Saos-2, MG-63) or bone tissue lysates.
• For ELISA, perform a dilution series to optimize the signal-to-noise ratio for your specific assay conditions.
• Given the confirmed cross-reactivity with mouse and rat, this antibody can be considered for rodent models in osteogenesis research.
• As with all polyclonal antibodies, lot-to-lot variability should be evaluated by testing each new batch in your experimental system.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers and research laboratories with a comprehensive range of IVD raw materials, including this anti-SP7 polyclonal antibody for bone biology and osteogenic differentiation studies. In addition to validated antibodies, we offer matched antibody pairs, bulk ancillary reagents, and sourcing services for rare targets to support your assay development from concept to clinic.