Anti-SP3 Rabbit Polyclonal Antibody for WB, IHC-P, ELISA - Q02447

Core Product Specifications and Parameters

Biological Background: SP3 Function and Localization

• SP3 (Transcription factor Sp3, SPR-2) is a zinc-finger transcription factor that can function as both an activator and repressor of gene expression, with its activity modulated by isoform selection and post-translational modifications.
• It specifically binds to GT and GC box promoter elements, thereby competing with SP1 for regulatory sites and finely tuning transcriptional output.
• Although classified as a weak activator, SP3 contributes to the regulation of diverse biological processes including cell-cycle progression, hormone-responsive pathways, and maintenance of housekeeping genes.
• SP3 localizes to the nucleus and PML nuclear bodies, consistent with its role in transcription regulation and potential involvement in nuclear signaling structures.
• The protein is ubiquitously expressed across human tissues, highlighting its broad regulatory importance in normal cellular physiology.
• Key post-translational modifications on SP3 include acetylation, phosphorylation, and ubiquitin-like conjugation (sumolylation), which critically influence its transcriptional activity and protein stability.

Experimental Guidance and Technical Tips

• For Western blot (WB), a specific band near 82 kDa is expected in cell lines expressing SP3; include nuclear extracts and negative controls to confirm target recognition.
• In immunohistochemistry (IHC-P), nuclear localization is expected; antigen retrieval parameters (e.g., citrate buffer pH 6.0, heat-induced epitope retrieval) should be optimized.
• For ELISA, consider pairing with another SP3-specific antibody for sandwich detection; validate sensitivity and specificity using recombinant SP3 protein or cell lysates.
• Cross-reactivity with mouse and rat has been reported; however, verify species-specific band sizes and expression patterns in your experimental system.

CamelBio: Your One-Stop Sourcing Bridge

At CamelBio, we streamline your transition from concept to clinic with a consolidated portfolio of IVD raw materials, including validated antibody pairs and optimized polyclonal/monoclonal antibodies for transcription factor targets like SP3. Whether you need bulk ancillary reagents or assistance sourcing rare targets, our team ensures reliable, regulatory-ready supply supported by comprehensive technical consultation.