Anti-SP2 Polyclonal Antibody for WB, ELISA - Q02086

Core Product Specifications and Parameters

Biological Background: SP2 Function and Localization

• SP2 is a zinc-finger transcription factor belonging to the Sp/KLF family, characterized by C2H2-type zinc finger domains that facilitate DNA binding.
• It selectively binds to GC-box promoter elements (5'-GGGCGG-3') and regulates transcription of target genes.
• SP2 functions primarily as a transcriptional activator, promoting mRNA synthesis from genes with functional GC-box recognition sites.
• The protein localizes to the nucleus, consistent with its role in transcriptional regulation.
• Alternative splicing of the SP2 gene generates multiple isoforms, potentially expanding its regulatory repertoire.
• SP2 is subject to phosphorylation, a post-translational modification that may modulate its activity, DNA binding, or protein interactions.
• As a metal-binding protein, its zinc finger motifs coordinate zinc ions essential for structural integrity and DNA recognition.

Experimental Guidance and Technical Tips

• The immunogen corresponds to amino acids 240-331 of human SP2, a region that may encompass part of the zinc finger domain or linker sequences; consider this when assessing isoform specificity.
• For Western blotting, the expected molecular weight is approximately 65 kDa; verify band size under reducing and non-reducing conditions, and note that post-translational modifications may cause slight shifts.
• This polyclonal antibody may recognize multiple SP2 isoforms or splice variants due to its raised nature; validate specificity using siRNA-mediated knockdown or SP2-negative cell lines if available.
• The antibody cross-reacts with mouse SP2, indicating epitope conservation; for mouse studies, confirm recognition of the endogenous protein in relevant tissue or cell lysates.
• In ELISA applications, use the recombinant immunogen as a positive control and consider coating conditions and blocking buffers to minimize background.
• Include appropriate controls (e.g., pre-immune serum, peptide blocking) to distinguish specific signals from non-specific binding typical of polyclonal preparations.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers comprehensive access to IVD raw materials, technical services, and consulting, supporting your journey from concept to clinic. For transcription factor research like SP2, we supply validated antibody pairs, optimized monoclonal and polyclonal antibodies, bulk ancillary reagents, and rare target sourcing. Let CamelBio streamline your procurement process and accelerate your diagnostic development.