Anti-SP110 Rabbit Polyclonal Antibody for WB and ELISA - Q9HB58

Core Product Specifications and Parameters

Biological Background: SP110 Function and Localization

• SP110, also known as interferon-induced protein 41/75 (IFI41/IFI75) and transcriptional coactivator Sp110, is a member of the speckled protein family.
• It functions as a transcription factor and putative nuclear hormone receptor coactivator, enhancing transcription of genes containing retinoic acid response elements (RARE).
• The protein localizes to the nucleus, consistent with its role in transcriptional regulation.
• SP110 is highly expressed in immune tissues such as peripheral blood leukocytes and spleen, with lower expression in other tissues including thymus, prostate, testis, ovary, small intestine, and colon.
• It contains key structural domains including a bromodomain and zinc-finger motifs, and is subject to post-translational modifications such as phosphorylation and alternative splicing.
• Keywords associated with SP110 include DNA-binding, host-virus interaction, and metal-binding, reflecting its diverse regulatory functions.

Experimental Guidance and Technical Tips

• For Western blotting, SP110 is expected to migrate at approximately 78 kDa (UniProt-predicted molecular weight of ~78.4 kDa). Validate detection in relevant cell lines or tissues such as peripheral blood leukocytes, spleen, or thymus.
• As a polyclonal antibody, lot-to-lot consistency may vary; it is advisable to validate each new lot in your specific assay system before large-scale experiments.
• For ELISA applications, optimize the coating antigen concentration and antibody dilution using a checkerboard titration to achieve the desired sensitivity and specificity.
• Always include appropriate positive and negative controls (e.g., lysates from cells with known SP110 expression levels) to confirm assay performance.
• Given the cross-reactivity with mouse and rat, this antibody can be considered for studies using these species, but initial validation in the target tissue is recommended.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers diagnostic manufacturers and research laboratories a streamlined, single-vendor solution for sourcing validated IVD raw materials. This anti-SP110 polyclonal antibody supports studies in transcription regulation, autoimmune disorders, and viral host interactions, complementing CamelBio’s portfolio of high-quality antibodies, antibody pairs, and ancillary reagents. Whether you require bulk quantities of this product, optimized monoclonal/polyclonal antibodies, or assistance in sourcing rare targets, CamelBio serves as your reliable procurement partner from concept to clinic.