Anti-SP100 Polyclonal Antibody for WB and ELISA - P23497

Core Product Specifications and Parameters

Biological Background: SP100 Function and Localization

• SP100 is a nuclear autoantigen and tumor suppressor, a major constituent of PML nuclear bodies, involved in cell growth, differentiation, and apoptosis.
• It functions as a transcriptional coactivator of ETS1 and ETS2, enhancing their transcriptional activity, but can also act as a corepressor of ETS1 under certain conditions, inhibiting its DNA binding. Related references: PMID:11909962, PMID:15247905
• SP100 plays a role in angiogenesis by regulating endothelial cell motility and invasion through ETS1.
• It interacts with the MRN complex to regulate telomere lengthening.
• SP100 may regulate TP53-mediated transcription and, through CASP8AP2, regulate FAS-mediated apoptosis.
• The protein localizes primarily to the nucleus, specifically to PML bodies and nuclear bodies, but can also be found in the cytoplasm.
• Widely expressed tissues, but isoform Sp100-B is restricted to spleen, tonsil, thymus, and certain B and T cell lines.
• Post-translational modifications include acetylation, phosphorylation, and isopeptide bond formation; it also features alternative splicing and a coiled-coil domain.

Experimental Guidance and Technical Tips

• The immunogen corresponds to amino acids 181-480 of human SP100, a central region that may be recognized by the polyclonal antibody in both human and mouse samples due to sequence conservation.
• For Western blot, expect bands around 100 kDa; however, alternative splicing isoforms may produce additional bands; validate with a known positive control such as HeLa cell lysate.
• In ELISA applications, this antibody can be used to detect recombinant or native SP100; optimize coating and detection conditions using the recombinant protein immunogen.
• Since SP100 is a nuclear protein, ensure proper nuclear extraction protocols for effective detection in WB.
• Cross-reactivity with mouse is reported; verify sequence homology of the immunogen region between human and mouse SP100 for quantitative applications.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers and research laboratories with one-stop access to IVD raw materials and technical services. This SP100 polyclonal antibody supports research into PML nuclear body biology, transcriptional regulation, and virus-host interactions. Our portfolio includes validated antibody pairs, optimized polyclonal and monoclonal antibodies, bulk ancillary reagents, and sourcing for rare targets to streamline your assay development.