Anti-SP1 Rabbit Polyclonal Antibody for WB, IHC-P, IF/ICC, ELISA - P08047

Core Product Specifications and Parameters

Biological Background: SP1 Function and Localization

• Transcription factor Sp1 is a zinc-finger protein that binds GC-rich promoter motifs to regulate a vast number of genes involved in cell growth, apoptosis, differentiation, and immune responses.
• It functions as both an activator and repressor depending on target gene context and post-translational modifications.
• The protein undergoes extensive post-translational modifications including phosphorylation, sumoylation, glycosylation, and acetylation, which modulate its activity, stability, and interactions.
• SP1 localizes primarily to the nucleus but can also be detected in the cytoplasm.
• It is implicated in chromatin remodeling and DNA damage response, and plays a role in protecting cells against oxidative stress.
• Positively regulates the transcription of the core clock component BMAL1, linking SP1 to circadian rhythm control. Related references: PMID:10391891 PMID:11371615 PMID:11904305
• In complex with ATF7IP, maintains telomerase activity in cancer cells by inducing TERT and TERC expression.
• Isoform 3 is a stronger transcriptional activator and is ubiquitously expressed at low levels, while SP1 is up-regulated in stomach adenocarcinomas.

Experimental Guidance and Technical Tips

• The immunogen corresponds to amino acids 420–620 of human SP1, covering a region that may be conserved across isoforms. The antibody is expected to detect full-length SP1 at ~81 kDa in Western blot.
• For immunohistochemistry (IHC-P), consider optimizing antigen retrieval conditions and validating the staining pattern in known positive tissues (e.g., stomach adenocarcinoma) and normal controls.
• The antibody is suitable for immunofluorescence (IF/ICC) to visualize nuclear localization of SP1; include appropriate fixation and permeabilization steps.
• In ELISA applications, use the recombinant protein immunogen as a positive control to establish assay sensitivity and specificity.
• Cross-reactivity with mouse and rat SP1 has been reported; validate consistency of detection in these species under your specific experimental conditions.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers diagnostic manufacturers, laboratories, and research institutes a comprehensive sourcing solution for IVD raw materials. From this anti-SP1 rabbit polyclonal antibody to validated antibody pairs, bulk ancillary reagents, and rare target reagents, we provide one-stop access to support your SP1-related transcription and cancer research projects. Our supply chain is designed to streamline your transition from concept to clinic with reliable, high-quality materials.