Anti-SP1 Rabbit Monoclonal Antibody for WB, IHC-P, IF, ChIP, CUT&Tag - P08047

Core Product Specifications and Parameters

Biological Background: Transcription factor Sp1 Function and Localization

• Transcription factor Sp1 (specificity protein 1) is a ubiquitously expressed transcription factor that can activate or repress gene expression in response to physiological and pathological stimuli.
• It binds with high affinity to GC-rich motifs (GC boxes) and regulates genes involved in cell growth, apoptosis, differentiation, and immune responses.
• Sp1 is highly regulated by post-translational modifications including phosphorylation, sumoylation, proteolytic cleavage, glycosylation, and acetylation.
• In complex with ATF7IP, Sp1 maintains telomerase activity in cancer cells by inducing TERT and TERC gene expression.
• Sp1 positively regulates the transcription of the core clock component BMAL1, playing a role in circadian rhythm regulation. Related references: PMID:10391891 PMID:11371615 PMID:11904305
• Sp1 primarily localizes to the nucleus but can also be found in the cytoplasm.
• It is implicated in chromatin remodeling, DNA damage response, and protection against oxidative stress following brain injury.
• Isoform 3 is a stronger activator of transcription than isoform 1; Sp1 is up-regulated in certain adenocarcinomas (e.g., stomach) at the protein level.

Experimental Guidance and Technical Tips

• For western blot, a band at approximately 81 kDa is expected; validate with appropriate lysates and consider the possibility of post-translational modifications influencing migration.
• This monoclonal antibody has been validated for ChIP, ChIP-seq, and CUT&Tag applications; ensure optimization of antibody concentration and crosslinking conditions for your specific experimental setup.
• Tested for cross-reactivity with human, mouse, and rat samples; verify in your own model system.
• Use appropriate negative controls (e.g., isotype-matched IgG) for immunoprecipitation-based assays.
• For immunofluorescence, Sp1 exhibits nuclear staining; confirm localization with DAPI co-stain.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers and research laboratories with reliable one-stop access to IVD raw materials, including this high-quality anti-SP1 rabbit monoclonal antibody for transcription and cancer research. Our comprehensive portfolio covers validated antibody pairs, optimized monoclonal and polyclonal antibodies, and bulk ancillary reagents, supporting your entire workflow from development to clinical application.