Anti-SOX9 Rabbit Monoclonal Antibody for WB - P48436

Core Product Specifications and Parameters

Biological Background: SOX9 Function and Localization

• Transcription factor SOX-9 is a member of the SOX (SRY-related HMG-box) family of transcription factors, encoded by the SOX9 gene in humans (Gene ID: 6662). Alternative names include CMD1, SRA1, CMPD1, SRXX2, and SRXY10.
• SOX9 plays a critical role in chondrocyte differentiation and skeletal development, specifically binding the 5'-ACAAAG-3' DNA motif in enhancers and super-enhancers to drive expression of cartilage matrix genes like COL2A1, COL4A2, COL9A1, COL11A2, ACAN, SOX5, and SOX6 (PubMed:8640233). Related references: PMID:8640233
• It is absolutely required for precartilaginous condensation, the first step in chondrogenesis, and together with SOX5 and SOX6, drives overt chondrogenesis and subsequent chondrocyte maturation.
• SOX9 blocks osteoblast differentiation by lowering beta-catenin (CTNNB1) signaling and RUNX2 expression, and promotes chondrocyte hypertrophy by transactivating COL10A1 with MEF2C.
• Low lipid levels promote SOX9 expression via FOXO transcription factors (FOXO1 and FOXO3), inducing chondrogenic commitment and suppressing fatty acid oxidation (PubMed:24038782). Related references: PMID:24038782
• Beyond cartilage, SOX9 regulates proliferation and differentiation in epithelial stem/progenitor cells, including lung branching morphogenesis and kidney development.
• SOX9 localizes to the nucleus and undergoes post-translational modifications such as acetylation, phosphorylation, and ubiquitination.
• Additional UniProt keywords include: 3D-structure, Disease variant, Isopeptide bond, Proteomics identification, Reference proteome, Transcription, Transcription regulation, Ubl conjugation.

Experimental Guidance and Technical Tips

• For western blotting, consider using whole cell lysates from human, mouse, or rat chondrocytes or other SOX9-expressing cell lines and validate signal at the expected molecular weight of 56 kDa.
• The immunogen covers the central region of human SOX9 (aa 169–300); verify cross-reactivity in mouse and rat samples as needed, and confirm specificity using appropriate controls.
• For immunohistochemistry (IHC-P) and immunofluorescence (IF/ICC), antigen retrieval and careful optimization of primary antibody concentration are recommended.
• This antibody is suitable for multiple applications including WB, IP, IF/ICC, IF-P, IHC-P, and ELISA; please refer to the supplier's recommended protocols and validate in your experimental system.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers diagnostic manufacturers and research institutes reliable, one-stop access to IVD raw materials, supporting projects from early R&D through clinical readiness. Our portfolio features high-quality antibodies for key transcription factors like SOX9, which are essential for skeletal development and chondrogenesis research. Reach out to explore validated antibody pairs, bulk ancillary reagents, and customized sourcing solutions for your assay development needs.