Anti-SOX5 Rabbit Monoclonal Antibody for WB, ELISA - P35711

Core Product Specifications and Parameters

Biological Background: SOX5 Function and Localization

• SOX5 is a transcription factor essential for chondrocyte differentiation and cartilage formation, binding specifically to the 5'-AACAAT-3' DNA motif.
• It promotes expression of cartilage matrix genes such as COL2A1 and AGC1 by acting on enhancers and super-enhancers.
• SOX5 cooperates with SOX6 and SOX9; together they bind enhancers to potentiate SOX9-mediated transactivation of cartilage-specific genes.
• Required for the transition from prechondrocytes to early chondrocytes, and for maintaining proliferating chondroblasts and columnar chondroblast formation.
• SOX5 delays chondrocyte prehypertrophy but later promotes hypertrophy, and delays terminal differentiation of chondrocytes.
• Also binds the MPZ gene promoter, suggesting a potential role in myelination.
• Subcellular localization is nuclear, consistent with its role as a DNA-binding transcription factor.
• Keywords include alternative splicing, phosphoprotein, and coiled coil, indicating regulatory complexity; mutations are associated with intellectual disability.

Experimental Guidance and Technical Tips

• The immunogen corresponds to the N-terminal region, so the antibody may recognize full-length SOX5 as well as isoforms containing this region.
• For Western blot, consider using nuclear extract as the sample source, given SOX5 nuclear localization.
• Validate antibody specificity in appropriate cell lines or tissues known to express SOX5, such as chondrocytes or cartilage.
• For ELISA, use standard peptide titration to establish sensitivity.
• Cross-reactivity with mouse and rat indicates conserved N-terminal sequence; confirm linear epitope recognition.
• Consider blocking with the immunizing peptide to confirm specificity in Western blot.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers, laboratories, and research institutes with one-stop access to IVD raw materials. For SOX5 and other transcription factors involved in skeletal development, we offer validated antibody pairs, optimized monoclonal and polyclonal antibodies, and bulk ancillary reagents to support your assay development and research needs.