Anti-SOX5 Polyclonal Antibody for WB, IHC-P, IF/ICC, ELISA - P35711

Core Product Specifications and Parameters

Biological Background: SOX5 Function and Localization

• Transcription factor SOX-5 specifically binds the 5'-AACAAT-3' DNA motif in enhancers and super-enhancers to promote chondrogenesis-related gene expression.
• Plays a critical role in chondrocyte differentiation and cartilage formation, acting downstream of precartilaginous condensation to drive early chondrocyte development.
• Cooperates with SOX6 and SOX9 on cartilage-specific gene enhancers, potentiating SOX9-mediated transactivation of matrix genes like COL2A1 and AGC1.
• Together with SOX6, maintains a proliferating chondroblast pool, regulates columnar chondroblast formation, delays prehypertrophy, and promotes hypertrophy while delaying terminal differentiation.
• Binds the proximal promoter region of the myelin protein MPZ gene, indicating potential roles beyond skeletal development.
• Subcellular localization is primarily nuclear, consistent with its function as a transcription regulator.
• Classified as an activator, DNA-binding protein, and phosphoprotein, and is subject to alternative splicing, yielding multiple isoforms including the L-SOX5 variants.
• Association with intellectual disability highlights its broader developmental significance beyond chondrogenesis.

Experimental Guidance and Technical Tips

• The immunogen is a synthetic peptide derived from the N-terminal region (aa 1-120), which may detect multiple SOX5 isoforms, including those generated by alternative splicing.
• For western blot, consider using nuclear extracts from chondrocyte-like cells or tissues such as cartilage to enrich the target protein, given its nuclear localization and expected molecular weight of 84 kDa.
• In immunohistochemistry/immunofluorescence, antigen retrieval may be necessary to expose nuclear epitopes; optimize fixation and permeabilization conditions for your particular sample type.
• ELISA applications may rely on the peptide immunogen; consider coating with the immunogen peptide or recombinant SOX5 fragments and validate linearity and sensitivity in your assay system.
• Cross-reactivity with mouse and rat has been validated by the supplier; for other species, perform sequence alignment of the immunogen region to predict recognition.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers comprehensive procurement support for SOX5 antibodies and related chondrogenesis research reagents, validated for IVD development. Our supply capabilities encompass monoclonal/polyclonal antibody pairs, bulk ancillary reagents, and customized sourcing for rare targets. Whether you need consistent lot-to-lot performance or scale-up support, CamelBio bridges your raw material needs from concept to clinic.