Anti-SOX4 Polyclonal Antibody for WB, ELISA - Q06945

Core Product Specifications and Parameters

Biological Background: SOX4 Function and Localization

• Transcription factor SOX-4 is a member of the SOX (SRY-related HMG-box) family of DNA-binding proteins, acting as a transcriptional activator.
• Binds with high affinity to the T-cell enhancer motif 5'-AACAAAG-3', implicating it in immune cell gene regulation. Related references: PMID:30661772
• Plays a critical role in the maturation and development of IL17A-producing Vγ2-positive γδ T-cells by modulating RORC expression (By similarity).
• Involved in skeletal myoblast differentiation by promoting CALD1 gene expression. Related references: PMID:26291311
• Localizes exclusively to the nucleus, consistent with its function as a transcription factor.
• Predominantly expressed in testis, brain, and heart, suggesting roles in neural and cardiac development.
• Post-translational modifications include acetylation, which may regulate its transcriptional activity.

Experimental Guidance and Technical Tips

• For Western blot, an expected band near 47 kDa may be observed. As a polyclonal antibody, lot-to-lot consistency should be titrated and validated in the relevant sample system.
• Given the immunogen's N-terminal peptide sequence (aa 1–100), the antibody may recognize full-length SOX4. Cross-reactivity with mouse suggests conservation of this epitope; consider this for interspecies studies.
• In ELISA, the antibody may be suitable for detecting recombinant or endogenous SOX4; pairing with a monoclonal antibody could improve specificity.
• Target expression is highest in testis, brain, and heart; these tissues could serve as positive controls, but expression levels should be verified under your experimental conditions.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is your comprehensive partner for IVD raw-material sourcing, providing validated antibody pairs, bulk ancillary reagents, and rare target materials to support your diagnostic and research workflows. This anti-SOX4 polyclonal antibody aligns with immune function and developmental biology applications, and we can assist with custom monoclonal development, assay-matched reagent bundles, and technical consultation to accelerate your project from concept to clinic.