Anti-SOX30 Rabbit Polyclonal Antibody for WB, ELISA - Q8CGW4

Core Product Specifications and Parameters

Biological Background: SOX30 Function and Localization

• SOX30 is a transcription factor belonging to the SOX (SRY-related HMG-box) family, encoded by the Sox30 gene.
• It acts both as a transcriptional activator and a repressor, binding to the DNA sequence 5´-ACAAT-3´ with a preference for surrounding guanine residues. Related references: PMID:29848638 PMID:29866902
• SOX30 binds to its own promoter, activates its own transcription, and is required for the expression of postmeiotic genes essential for spermiogenesis. Related references: PMID:29848638 PMID:29866902
• It inhibits Wnt signaling by repressing CTNNB1 transcription and by directly binding to CTNNB1 protein, preventing its interaction with TCF7L2/TCF4. Related references: PMID:29739711
• SOX30 localizes to both the nucleus and the cytoplasm.
• Tissue-specific expression includes lung (at protein level) and testis, with detailed detection in preleptotene spermatocytes, round spermatids, elongated spermatids, and pachytene spermatocytes during spermatogenesis stages; also expressed in ovaries. Related references: PMID:10359848 PMID:25609838 PMID:29739711 PMID:29848638 PMID:29866902

Experimental Guidance and Technical Tips

• The immunogen corresponds to the N-terminal region (amino acids 1-150) of mouse SOX30; the resulting polyclonal antibody may recognize full-length SOX30 and potentially N-terminal isoforms. Validate specificity in your system using appropriate controls.
• For Western blot (WB), the expected molecular weight is approximately 84 kDa. Consider using testis or lung tissue lysates as positive controls, as SOX30 is known to be expressed in these tissues.
• For ELISA, the antibody is applicable; optimize coating antigen concentration and antibody dilution based on your specific assay conditions.
• When performing experiments, ensure proper validation of cross-reactivity with human, mouse, and rat samples as indicated.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves as a comprehensive B2B sourcing partner for IVD raw materials, providing validated antibody pairs, polyclonal and monoclonal antibodies, and ancillary reagents. This anti-SOX30 polyclonal antibody supports research in transcription regulation and spermatogenesis, aligning with your needs from early development through to clinical application.