Anti-SOX30 Rabbit Polyclonal Antibody for WB, ELISA - O94993

Core Product Specifications and Parameters

Biological Background: SOX30 Function and Localization

• Transcription factor SOX-30 is a member of the SOX family of HMG-box proteins that play critical roles in development and differentiation.
• SOX-30 functions as both a transcriptional activator and repressor. It binds specifically to the 5’-ACAAT-3’ DNA motif with a preference for flanking guanine residues. Related references: PMID:10359848
• The protein can bind to its own promoter and activate its own transcription, indicating an autoregulatory loop.
• SOX-30 is essential for spermiogenesis, where it activates the expression of postmeiotic genes required for sperm development.
• In the Wnt signaling pathway, SOX-30 binds the CTNNB1 promoter to repress transcription and directly interacts with the CTNNB1 protein, preventing its association with TCF7L2/TCF4 and thereby inhibiting Wnt signaling. Related references: PMID:29739711
• Subcellular localization is primarily nuclear, consistent with its role as a transcription factor, but it can also be detected in the cytoplasm.
• The protein has a calculated molecular weight of approximately 81.8 kDa, and migrates at ~82 kDa in SDS-PAGE. Alternative splicing may produce multiple isoforms.

Experimental Guidance and Technical Tips

• The immunogen sequence (amino acids 604-753) spans a C-terminal region of human SOX30. This region may confer specificity; validation with relevant controls is recommended.
• For Western blotting, a band around 82 kDa is expected. The antibody has been tested on human, mouse, and rat samples; consider optimizing loading and detection conditions for your model system.
• In ELISA, titration of both antibody and antigen is advised to establish optimal working concentrations.
• Since SOX30 has dual roles in transcriptional regulation and Wnt signaling, consider using appropriate nuclear/cytoplasmic extracts or pathway-specific controls in your assays.

CamelBio: Your One-Stop Sourcing Bridge

For researchers and diagnostic developers working on SOX30-dependent transcription, spermiogenesis, or Wnt pathway modulation, CamelBio provides reliable access to validated antibodies and ancillary reagents. We offer this anti-SOX30 polyclonal antibody in bulk quantities, alongside optimized matched pairs, recombinant proteins, and buffer systems to support your workflow. Through our one-stop sourcing model, CamelBio simplifies procurement of rare and standard IVD raw materials, helping you accelerate from concept to clinic.