Anti-SOX2 Rabbit PolymAb® for WB, IP, IF, IHC, ChIP, ELISA - P48431

Core Product Specifications and Parameters

Biological Background: SOX2 Function and Localization

• SOX2 is a transcription factor that critically regulates early embryogenesis and embryonic stem cell pluripotency.
  Related references: PMID:18035408
• Forms a trimeric complex with OCT4 on DNA, controlling genes such as YES1, FGF4, UTF1, and ZFP206.
• Binds the proximal enhancer region of NANOG, contributing to pluripotency maintenance.
• Acts downstream of SRRT to promote neural stem cell self-renewal and keeps neural cells undifferentiated by counteracting proneural proteins.
• Localizes to the nucleus and nucleus speckles, but can also be detected in the cytoplasm.
• Post-translational modifications include phosphorylation, methylation, and isopeptide bond formation (Ubl conjugation), as indicated by UniProt keywords.
• Mutations in SOX2 are associated with microphthalmia syndromes, reflected by its aliases ANOP3 and MCOPS3.

Experimental Guidance and Technical Tips

• Synthetic peptide immunogen design indicates epitope-specific detection; validate in your experimental context.
• Reported applications include WB, IP, IF/ICC, IF-P, IHC-P, ChIP, and ELISA; optimization of conditions is recommended.
• Expected to react with human, mouse, and rat; confirm cross-reactivity if using in other species.
• Nuclear localization of SOX2 suggests nuclear extraction or fixation/permeabilization for IF/IHC may be necessary.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves as a strategic sourcing partner for IVD manufacturers and research labs, offering validated antibody pairs, optimized monoclonal and polyclonal antibodies, and bulk ancillary reagents. For transcription factor targets like SOX2, we also facilitate rare target raw-material sourcing and provide technical consulting to support your assay development pipeline.