Anti-SOX2 Polyclonal Antibody for WB, IHC-P, IF/ICC, IP, ELISA - P48431

Core Product Specifications and Parameters

Biological Background: SOX2 Function and Localization

• Transcription factor SOX2 (SRY-box transcription factor 2) forms a trimeric complex with OCT4 on DNA, controlling the expression of genes such as YES1, FGF4, UTF1, and ZFP206 involved in embryonic development (By similarity).
• It binds to the proximal enhancer region of NANOG and is critical for early embryogenesis and embryonic stem cell pluripotency. Related references: PMID:18035408
• As a downstream target of SRRT, SOX2 promotes neural stem cell self-renewal and maintains neural cells in an undifferentiated state by counteracting the activity of proneural proteins and suppressing neuronal differentiation (By similarity).
• The protein localizes to the nucleus, nuclear speckles, and cytoplasm.
• Post-translational modifications include phosphorylation, methylation, isopeptide bond formation, and ubiquitin-like conjugation.
• SOX2 is a developmental protein and transcriptional activator, involved in transcription regulation and DNA binding.
• Mutations in SOX2 are associated with congenital anomalies including anophthalmia and microphthalmia.

Experimental Guidance and Technical Tips

• The immunogen (aa 1–140 of human SOX2) targets the N-terminal region; consider that this may detect full-length as well as N-terminal fragments or isoforms.
• For western blotting, the predicted molecular weight is 34 kDa; optimize gel percentage and transfer conditions accordingly.
• In immunohistochemistry (IHC) and immunofluorescence (IF), validate antigen retrieval methods and include appropriate controls, noting that SOX2 can localize to the nucleus and cytoplasm.
• For immunoprecipitation (IP), ensure antibody compatibility with your lysis buffer and consider that SOX2 may be in complex with other proteins.
• Cross-reactivity with human, mouse, and rat has been reported; validate in your specific tissue or cell type.
• Determine optimal working dilution empirically for each application.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio stands as your comprehensive one-stop sourcing partner for IVD raw materials, providing access to validated antibody pairs, optimized polyclonal antibodies like this anti-SOX2 product, and a wide range of ancillary reagents to support stem cell and developmental biology research. Whether you need rare target raw materials or bulk supply, our integrated sourcing capabilities bridge your journey from concept to clinic.