Anti-SOX2 Monoclonal Antibody for WB, IF-F, ELISA - P48431

Core Product Specifications and Parameters

Biological Background: SOX2 Function and Localization

• SOX2 is a transcription factor that forms a trimeric complex with OCT4 on DNA, controlling expression of genes like YES1, FGF4, UTF1, and ZFP206 involved in embryonic development.
• It binds to the proximal enhancer region of NANOG, a key pluripotency factor, and is critical for early embryogenesis and embryonic stem cell pluripotency. Related references: PMID:18035408
• SOX2 acts downstream of SRRT to promote neural stem cell self-renewal and keeps neural cells undifferentiated by counteracting proneural proteins, suppressing neuronal differentiation.
• It may function as a developmental switch in neuronal differentiation.
• Alternative names for SOX2 include ANOP3 and MCOPS3, associated with microphthalmia and other developmental disorders (disease variant keyword).
• Subcellular localization includes nucleus speckles, cytoplasm, and nucleus, and the protein is subject to post-translational modifications such as phosphorylation, methylation, and ubiquitination (Ubl conjugation).
• The human SOX2 protein has a molecular weight of approximately 34 kDa (34310 Da from UniProt).

Experimental Guidance and Technical Tips

• The immunogen is a synthetic peptide corresponding to the N-terminal region (aa 1-100) of human SOX2. For Western blot, a band around 34 kDa is expected; consider using positive controls like human embryonic stem cell lysates.
• For immunofluorescence (IF-F), fixation and permeabilization conditions may need optimization to detect nuclear-localized SOX2; validate in relevant cell lines.
• Cross-reactivity with mouse and rat has been reported; however, we recommend confirming species-specific reactivity in your experimental setup.
• For ELISA, pair with an appropriate capture/detection antibody and validate using recombinant SOX2 protein or cell lysates.
• Given the role of SOX2 in stem cell pluripotency, this antibody is suitable for developmental biology and stem cell research applications.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers and research laboratories with reliable access to high-quality IVD raw materials, including validated monoclonal antibodies like this anti-SOX2 antibody for stem cell and developmental biology applications. Our supply capabilities encompass antibody pairs, bulk reagents, and custom sourcing for rare targets—supporting your projects from concept to clinic.