Anti-SOX18 Polyclonal Antibody for WB, ELISA - P35713

Core Product Specifications and Parameters

Biological Background: SOX18 Function and Localization

• SOX18 is a member of the SOX (SRY-related HMG-box) family of transcription factors, characterized by a conserved HMG-box DNA-binding domain.
• It functions as a transcriptional activator, recognizing the consensus sequence 5'-AACAAAG-3' in target gene promoters.
• Essential for embryonic cardiovascular development and lymphangiogenesis; activates expression of PROX1 and other lymphatic endothelial markers.
• Triggers differentiation of lymphatic vessels, but is not required for the maintenance of differentiated lymphatic endothelial cells.
• Plays a functionally redundant role with SOX17 in postnatal angiogenesis.
• Interaction with MEF2C enhances its transcriptional activation activity.
• Required for normal hair development, and mutations are associated with hypotrichosis-lymphedema-telangiectasia syndrome (HLTS).
• Subcellularly localized to the nucleus, with tissue expression detected in heart, lung, placenta, skeletal muscle, liver, kidney, spleen, prostate, ovary, small intestine, and colon.

Experimental Guidance and Technical Tips

• The immunogen corresponds to the N-terminal region (aa 1-100) of human SOX18; sequence alignment across species of interest should be performed to validate epitope conservation if cross-reactivity is critical.
• For WB, the predicted molecular weight is approximately 41 kDa; appropriate positive controls such as endothelial or lymphatic cell lysates may be considered.
• As a polyclonal antibody, lot-to-lot consistency should be verified in your experimental system, and specificity confirmed with appropriate negative controls.
• ELISA applications may require optimization of coating antigen concentration and antibody dilution to achieve the desired signal-to-noise ratio.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers comprehensive IVD raw-material sourcing for diagnostic and life science laboratories, covering targets such as SOX18 involved in cardiovascular development, lymphangiogenesis, and hair disorders. Our supply portfolio includes validated antibody pairs, optimized polyclonal and monoclonal antibodies, bulk ancillary reagents, and rare target raw-material procurement. We act as a bridging partner, connecting your research and development needs with reliable, quality-assured immunoreagents.