Anti-SOX13 Polyclonal Antibody for WB, ELISA - Q9UN79

Core Product Specifications and Parameters

Biological Background: SOX13 Function and Localization

• SOX13 is a transcription factor that binds to the consensus DNA sequence 5’-AACAAT-3’, regulating gene expression. Related references: PMID:10871192
• Acts as a transcriptional repressor of myelin-related genes (MPZ, MBP) and may influence oligodendrocyte development.
• Inhibits WNT signaling by interacting with TCF7/TCF1, modulating gamma-delta T-cell differentiation and IL17A expression. Related references: PMID:20028982
• Promotes brown adipocyte differentiation through transcriptional regulation.
• Subcellular localization is both nuclear and cytoplasmic, reflecting its dynamic regulatory roles.
• Widely expressed in tissues including pancreas (exocrine cells and islets), placenta, kidney, brain, heart, lung, liver, and reproductive organs; also an autoantigen in type 1 diabetes. Related references: PMID:10871192 PMID:20028982
• Classified as a phosphoprotein, suggesting regulation by phosphorylation.
• Alternative names include Islet cell antigen 12 and Type 1 diabetes autoantigen ICA12, highlighting immunological relevance.

Experimental Guidance and Technical Tips

• The immunogen covers the N-terminal region (aa 1-180). Consider verifying reactivity in systems expressing full-length SOX13.
• For WB, confirm a band around the predicted 69 kDa molecular weight; additional bands may arise from phosphorylation or isoforms.
• In ELISA, use the recombinant protein (NP_005677.2 fragment) as a positive control and validate with cell lysates from target tissues (e.g., pancreas, brain) to ensure specificity.
• Cross-reactivity with mouse and rat allows use in orthologous models, but confirm sequence similarity in the target epitope before designing experiments.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers and research institutes with a comprehensive range of IVD raw materials, including validated antibody pairs, optimized polyclonal antibodies, and bulk ancillary reagents. For studies on transcription factors like SOX13, we offer reliable sourcing of rare-target antibodies and can support your project from concept to clinic. Contact us for one-stop procurement and technical consulting tailored to your diagnostic assay development.