Anti-SOX13 Polyclonal Antibody for WB and ELISA - Q9UN79

Core Product Specifications and Parameters

Biological Background: SOX13 Function and Localization

• SOX13 (also known as islet cell antigen 12, ICA12) is a member of the SOX family of transcription factors and a type 1 diabetes autoantigen.
• SOX13 binds to the DNA consensus sequence 5'-AACAAT-3'. Related references: PMID:10871192
• It acts as a transcriptional repressor for MPZ and MBP gene promoters, potentially involved in oligodendroglia differentiation.
• SOX13 inhibits WNT signaling by modulating TCF7/TCF1 activity, impacting gamma-delta T-cell development and IL17A expression. Related references: PMID:20028982
• SOX13 promotes brown adipocyte differentiation.
• SOX13 localizes to both the nucleus and cytoplasm, indicating nucleocytoplasmic shuttling.
• SOX13 is expressed in the exocrine pancreas, islets of Langerhans, and multiple other tissues including placenta, kidney, brain, heart, lung, liver, adipose tissue, cervix, colon, esophagus, ovary, prostate, small intestine, spleen, testicle, thymus, and thyroid. Related references: PMID:10871192, PMID:20028982
• Post-translational modifications include phosphorylation; the protein is classified as a DNA-binding repressor and a member of the reference proteome.

Experimental Guidance and Technical Tips

• The immunogen corresponds to amino acids 525-600 in the C-terminal domain of human SOX13. When performing WB, anticipate a band around 69 kDa; consider verifying specificity with overexpression or knockdown models.
• For ELISA applications, consider using recombinant SOX13 protein as a standard and include appropriate blocking and washing steps to minimize background.
• Cross-reactivity is reported for human, mouse, and rat. Validate the antibody's reactivity in your specific cell lines or tissue lysates, as target abundance and post-translational modifications may vary.
• Given the polyclonal nature, the antibody may recognize multiple epitopes, which can be advantageous for detecting denatured proteins in WB.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio fast-tracks your IVD development by providing a complete range of raw materials, from validated antibodies like this anti-SOX13 polyclonal to antibody pairs, bulk ancillary reagents, and rare‑target sourcing. Whether you're developing transcriptome or diabetes assays, our ready‑to‑ship inventory and flexible supply solutions help you bridge the gap from concept to clinic efficiently.