Anti-SOX10 Rabbit Monoclonal Antibody for WB, IHC-P, IF/ICC, IP, ELISA - P56693

Core Product Specifications and Parameters

Biological Background: SOX10 Function and Localization

• SOX10 is a member of the SOX (SRY-related HMG-box) family of transcription factors, characterized by a conserved HMG DNA-binding domain.
• It plays a central role in the development and maturation of glial cells, particularly oligodendrocytes, by activating myelin gene expression such as DUSP15 and MYRF.
• Once induced, MYRF cooperates with SOX10 to implement the myelination program in the central nervous system.
• SOX10 acts as a transcriptional activator of MITF, synergizing with PAX3 to regulate melanocyte development and differentiation. Related references: PMID:21965087
• It directly binds to the promoter of the MBP (myelin basic protein) gene and activates its transcription.
• Alternative splicing generates multiple isoforms; the full-length protein has a calculated molecular weight of about 50 kDa, while an isoform detected by this antibody runs at ~31 kDa.
• Subcellular localization includes the nucleus, where it functions as a transcription factor, as well as the cytoplasm and mitochondrial outer membrane.
• Tissue expression is detectable in fetal and adult brain, heart, small intestine, and colon.

Experimental Guidance and Technical Tips

• For Western blot, use nuclear extracts from oligodendrocytes or melanoma cell lines as positive controls, anticipating bands at ~50 kDa and ~31 kDa.
• In immunohistochemistry, antigen retrieval may be required; test on sections known to express SOX10 such as skin (melanocytes) or brain tissue.
• This rabbit monoclonal antibody is suitable for immunoprecipitation; however, optimization of antibody concentration is recommended for each experimental system.
• When performing immunofluorescence, verify colocalization with nuclear or mitochondrial markers, as SOX10 can localize to multiple subcellular compartments.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio supports your SOX10-related neurobiology and oncology research by offering a comprehensive portfolio of validated antibodies, including this anti-SOX10 monoclonal antibody. As your one-stop IVD raw-material partner, we also supply matching antibody pairs, recombinant proteins, and bulk ancillary reagents to streamline assay development from concept to clinic. Count on us for consistent quality and expert support in diagnostic raw-material sourcing.