Anti-SOX1 Polyclonal Antibody for WB, IHC-P, ELISA - O00570

Core Product Specifications and Parameters

Biological Background: SOX1 Function and Localization

• SOX1 (SRY-box 1) is a member of the SOX family of transcription factors characterized by a high-mobility-group (HMG) DNA-binding domain.
• SOX1 functions as a transcriptional activator and is a critical regulator of neuronal development.
• It acts as a molecular switch to maintain neural progenitor cells in an undifferentiated state by counteracting proneural protein activity.
• SOX1 suppresses premature neuronal differentiation, thereby preserving the neural stem cell pool during central nervous system development.
• The protein localizes exclusively to the nucleus, consistent with its role as a transcription factor.
• It is predominantly expressed in the developing central nervous system, reflecting its tissue-specific developmental function.
• SOX1 is classified under DNA-binding proteins, transcriptional activators, and reference proteome categories, highlighting its fundamental role in gene regulation.

Experimental Guidance and Technical Tips

• The immunogen corresponds to a C-terminal peptide (aa 292-391) of human SOX1; sequence homology with mouse and rat supports the reported cross-reactivity.
• For Western blot (WB), expect a band near 39 kDa; optimize blocking and antibody dilution using lysates from neural cell lines or embryonic neural tissues.
• In immunohistochemistry (paraffin) (IHC-P), nuclear staining is anticipated; heat-induced epitope retrieval (e.g., citrate buffer, pH 6.0) may improve signal.
• As a polyclonal antibody, lot-to-lot variability can occur; always validate in your specific experimental system.
• For ELISA, use peptide or recombinant SOX1 protein as coating antigen; titrate the antibody to determine optimal working concentration.
• Include appropriate positive and negative controls (e.g., SOX1-overexpressing vs. knockdown cells) to confirm specificity.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers, laboratories, and research institutes with streamlined access to a broad portfolio of IVD raw materials. The SOX1 polyclonal antibody supports neural development and oncology research, enabling robust assay development. Beyond single reagents, CamelBio offers validated antibody pairs, bulk ancillary reagents, and customized sourcing for rare targets, helping you accelerate from concept to clinic.