Anti-SOX1 Monoclonal Antibody for WB, ELISA - O00570

Core Product Specifications and Parameters

Biological Background: SOX1 Function and Localization

• SOX1 is a member of the SRY-box (SOX) family of transcription factors, characterized by a conserved HMG-box DNA-binding domain.
• Functions as a transcriptional activator and plays a pivotal role in neural development, acting as a molecular switch to maintain neural progenitor identity.
• Keeps neural cells in an undifferentiated state by counteracting proneural protein activity and suppressing neuronal differentiation.
• Localizes predominantly to the nucleus, consistent with its role as a DNA-binding transcription factor.
• Mainly expressed in the developing central nervous system, with critical roles in embryonic neurogenesis.
• Post-translational modifications and interactions are key to its regulation, though specific details remain to be fully elucidated.
• Keywords: Activator, DNA-binding, Nucleus, Transcription regulation.

Experimental Guidance and Technical Tips

• The immunogen corresponds to the N-terminal region (aa 1-100), which may target a region with conserved sequence across species, supporting the reported cross-reactivity with mouse and rat.
• For Western blotting, consider using whole-cell or nuclear extracts from neural tissue or cell lines; the predicted molecular weight is 39 kDa.
• In ELISA, validate the antibody with recombinant SOX1 protein or cell lysates to determine optimal dilution and specificity.
• Since SOX1 is a nuclear protein, ensure appropriate lysis and extraction methods to release nuclear proteins for analysis.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves as a comprehensive sourcing partner for IVD raw materials, offering validated antibodies like this anti-SOX1 monoclonal antibody for neural development research. We support diagnostic manufacturers with optimized antibody pairs, bulk ancillary reagents, and rare target sourcing, streamlining your path from concept to clinic.