Anti-SOSTDC1 Polyclonal Antibody for WB, ELISA - Q6X4U4

Core Product Specifications and Parameters

Biological Background: SOSTDC1 Function and Localization

• SOSTDC1 (Sclerostin domain-containing protein 1), also known as Ectodin, USAG-1 (uterine sensitization-associated gene 1 protein), DAND7, and CDA019, is a secreted protein belonging to the sclerostin family. It is expressed as a glycoprotein containing a cystine-knot motif and intramolecular disulfide bonds.
• It functions as a direct antagonist of bone morphogenetic proteins BMP2, BMP4, BMP6, and BMP7 in a dose-dependent manner, thereby modulating BMP signaling.
• Additionally, SOSTDC1 enhances Wnt signaling and inhibits TGF-beta signaling, implicating it in the regulation of multiple developmental pathways.
• May play a role in endometrial receptivity for implantation and sensitization for the decidual cell reaction (by similarity).
• Subcellular localization: Secreted. It is highly expressed in the kidney and weakly in the lung, suggesting tissue-specific regulation.
• Post-translational modifications include glycosylation and disulfide bond formation, and multiple isoforms may arise via alternative splicing.

Experimental Guidance and Technical Tips

• The immunogen used for this antibody is a recombinant protein spanning amino acids 115–206 of human SOSTDC1 (NP_056279.1). Consider sequence homology with intended species when designing experiments.
• For Western blot, the predicted molecular weight is ~23 kDa; glycosylation may shift the observed band. Use kidney lysates as a positive control given high expression.
• In ELISA, ensure proper coating of recombinant protein or native antigen and assess cross-reactivity with related BMP antagonists if needed.
• As a rabbit polyclonal antibody, it may provide signal amplification but consider potential batch-to-batch variability. Include appropriate secondary detection controls.
• Tested species: human, mouse, rat. For other species, verify epitope conservation before use.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is your integrated partner for IVD raw-material procurement, offering validated antibodies, recombinant proteins, and ancillary reagents. This anti-SOSTDC1 polyclonal antibody supports your studies in BMP/Wnt signaling and reproductive biology. With end-to-end sourcing and technical support from concept to clinic, CamelBio accelerates your diagnostic development.