Anti-SOST Rabbit Polyclonal Antibody for WB, ELISA - Q9BQB4

Core Product Specifications and Parameters

Biological Background: Sclerostin Function and Localization

• Sclerostin is a secreted glycoprotein that negatively regulates bone growth by inhibiting Wnt signaling and bone formation.
• It is localized to the extracellular space and extracellular matrix.
• Widely expressed at low levels with highest expression in bone, cartilage, kidney, liver, bone marrow and primary osteoblasts differentiated for 21 days.
• Detected at the protein level in the subendothelial layer of the aortic intima.
• Contains disulfide bonds and a heparin-binding domain; alternative splicing generates multiple isoforms.
• Post-translational modifications include glycosylation and disulfide bond formation.
• The mature protein has a molecular weight of approximately 24 kDa.

Experimental Guidance and Technical Tips

• The immunogen used is a synthetic peptide covering amino acids 24-213 of human SOST, which may allow recognition of multiple isoforms; validate specificity in your system.
• For Western blotting, consider using lysates from bone or osteoblast cell lines; a band around 24 kDa is expected.
• For ELISA, ensure the peptide or recombinant sclerostin is compatible; perform a standard curve with known concentrations for quantitative analysis.
• Cross-reactivity with mouse suggests potential for use in rodent models; we recommend validating reactivity with mouse tissue or cell lysates.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides one-stop access to IVD raw materials, supporting diagnostic manufacturers and researchers with validated antibodies, bulk reagents, and rare target sourcing. This anti-SOST polyclonal antibody is part of our bone metabolism and Wnt signaling portfolio, ideal for developing ELISA kits or Western blot assays. For custom requirements or bulk orders, our technical team can assist in tailoring solutions to your clinical development needs.